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Restriction Enzymes

Components	10X OPTIZYME™ Buffer 4
Cut Site	T.CTAGA
Form	Liquid
Product Type	XbaI
Storage Buffer	10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme

Modifying Enzymes

Content And Storage	-20°C
Form	Liquid
Product Type	T4 DNA Polymerase
Storage Buffer	OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
Color	Colorless
pH	7.5
Concentration	5 U/μL
For Use With (Application)	Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
Source	E. coli cells with a cloned gene 43 of bacteriophage T4

Restriction Enzymes

Restriction Enzymes

Restriction Enzymes

Restriction Enzymes

Restriction Enzymes

Restriction Enzymes

Modifying Enzymes

Content And Storage	-20°C
Form	Liquid
Product Type	T4 DNA Ligase
Storage Buffer	OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)
Color	Colorless
pH	7.5
Concentration	5 U/μL
For Use With (Application)	Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
Source	E. coli cells with a cloned gene 30 of bacteriophage T4

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