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| Gene Symbols | TP53BP1 |
|---|---|
| Gene Alias | 53BP1, p202, p53-binding protein 1, p53bp1, TDRD30, TP53-Binding Protein 1, tumor protein 53-binding protein, 1, tumor protein p53 binding protein 1, tumor protein p53-binding protein, 1, tumor suppressor p53-binding protein 1 |
Rabbit IgG Isotype Control, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 36 publications
Histone H2AX, p Ser139 Antibody (3F2), Novus Biologicals™
Mouse Monoclonal Antibody has been used in 6 publications
| Content And Storage | Store at -20°C. Avoid freeze/thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | Western Blot |
| Form | Purified |
| Isotype | IgG1 κ |
| Gene Accession No. | P16104 |
| Research Discipline | Checkpoint signaling, DNA Double Strand Break Repair, DNA Repair, Epigenetics, Mitotic Regulators, Phospho Specific |
| Concentration | 1 mg/mL |
| Antigen | Histone H2AX (p Ser139) |
| Gene Symbols | H2AFX |
| Regulatory Status | RUO |
| Purification Method | Protein G purified |
| Dilution | Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 1 μg 106 cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 4 μg/mL, Immunohistochemistry-Paraffin 1:10 - 1:500 |
| Molecular Weight of Antigen | 15 kDa |
| Gene Alias | H2A.X, H2A/X, H2AFX |
| Gene ID (Entrez) | 3014 |
| Immunogen | This Histone H2AX [p Ser139] Antibody (3F2) was developed against a synthetic peptide sequence surrounding phosphorylated Ser139. |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Test Specificity | In Western blot this antibody detects ∼17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate. In immunofluorescence procedures, recognizes phosphorylated H2AX in gamma irradiated HeLa cells. ELISA of phosphorylated H2AX can also be performed. Used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section. |
| Clone | 3F2 |
| Purity or Quality Grade | >97% pure by SDS-PAGE and HPLC |
|---|---|
| Gene Alias | Immunoglobulin G binding protein A, SPA, Staphylococcal protein A |
| Molecular Weight (g/mol) | 33.4 kDa |
| Gene ID (Entrez) | 3919448 |
| Formulation | Lyophilized from additive free solution. |
| Research Category | Epitope Tags |
| Reconstitution | Dissolve in distilled water or saline. |
| Endotoxin Concentration | Less than 0.1 EU/ug of Protein A as determined by LAL method. |
| Storage Requirements | Store at -20°C to -70°C as supplied. After reconstitution, store at 2°C to 8°C for 1 month and at -20°C to -70°C for long term storage. Avoid repeated freeze-thaw cycles. |
| Concentration | LYOPH |
| For Use With (Application) | PAGE,Bioactivity,HPLC |
| Protein | Protein A |
LC3B Antibody - BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 1218 publications
| Content And Storage | Store at -20°C. |
|---|---|
| Target Species | Human,Mouse,Rat,Pig,Alligator,Avian,Bacteria,Bovine,Canine,Chicken,Guinea Pig,Hamster,Invertebrate,Monkey,Primate,Rabbit,Golden Syrian Hamster,Zebrafish |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | ChIP Assay,ELISA,Flow Cytometry,Immunoassay,Immunoblot,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Immunoprecipitation,In situ PLA,KnockDown |
| Isotype | IgG |
| Gene Accession No. | Q9GZQ8 |
| Research Discipline | Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Mitophagy, Neuroscience |
| Concentration | 1.0 mg/mL |
| Antigen | LC3B |
| Gene Symbols | MAP1LC3B |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 0.5 - 2.0 ug/mL, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:200 - 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 20 ug/500 ug of protein, Immunohistochemistry-Paraffin 1:200 - 1:400, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated |
| Molecular Weight of Antigen | 14.688 kDa |
| Gene Alias | anti-LC3B, ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B |
| Gene ID (Entrez) | 81631 |
| Formulation | PBS with 0.02% Sodium Azide |
| Immunogen | Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8] |
| Classification | Polyclonal |
| Primary or Secondary | Primary |
| Test Specificity | Human, rat, mouse, zebrafish, bacteria, hamster, and porcine. Bovine reactivity reported in scientific literature (PMID: 24895572) Primate reactivity reported in scientific literature (PMID: 25142602) The mouse detection has been reported to be weaker than the human. Immunogen sequence has 84% homology to Xenopus. |
| Antigen | Ferroportin/SLC40A1 |
|---|---|
| Target Species | Human,Mouse,Pig,Bovine |
| Host Species | Rabbit |
| Applications | Western Blot,Immunohistochemistry (Paraffin) |
Separase Antibody (XJ11-1B12) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 1 publication
| Content And Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | Western Blot |
| Form | Purified |
| Isotype | IgG1 κ |
| Gene Accession No. | Q14674 |
| Research Discipline | Apoptosis, Cancer, Cell Biology, Cell Cycle and Replication, Cellular Markers, Chromatin Research, DNA Repair, Mitotic Regulators |
| Concentration | 0.9 mg/mL |
| Antigen | Separase |
| Gene Symbols | ESPL1 |
| Regulatory Status | RUO |
| Purification Method | Protein G purified |
| Dilution | Western Blot 1:500 |
| Gene Alias | Caspase-like protein ESPL1, EC 3.4.22.49, ESP1extra spindle poles like 1, extra spindle pole bodies homolog 1 (S. cerevisiae), extra spindle poles like 1 (S. cerevisiae), Extra spindle poles-like 1 protein, FLJ46492, KIAA0165separin, Separase |
| Gene ID (Entrez) | 9700 |
| Immunogen | Maltose-Binding Protein fusion of a C-terminal fragment of human Separase (residues 1866-1996). [UniProt# Q14674] |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Clone | XJ11-1B12 |
Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 647, Novus Biologicals™
Mouse Monoclonal Antibody
FANCD2 Antibody - BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 178 publications
| Content And Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Human,Mouse,Rat,Avian,Canine,Kangaroo,Primate,Zebrafish |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | Western Blot,ChIP Assay,Flow Cytometry,Immunohistochemistry,Immunocytochemistry,Immunoprecipitation |
| Isotype | IgG |
| Gene Accession No. | Q9BXW9 |
| Research Discipline | Breast Cancer, Cancer, DNA Double Strand Break Repair, DNA Repair, Epigenetics, Genes Sensitive to DNA Damaging Agents |
| Concentration | 1.0 mg/mL |
| Antigen | FANCD2 |
| Gene Symbols | FANCD2 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:10000-1:20000, Simple Western 1:25, Chromatin Immunoprecipitation reported in scientific literature (PMID 28196964), Flow Cytometry, Immunohistochemistry 2.5-5.0 ug/ml, Immunocytochemistry/Immunofluorescence 1:200-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 2.5-5.0 ug/ml, Immunoblotting reported in multiple pieces of scientific literature, RNA Inhibition reported in scientific literature (PMID 27694619), Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated |
| Molecular Weight of Antigen | 164.1 kDa |
| Gene Alias | DKFZp762A223, FA4, FACD, FAD, FAD2, FA-D2, FADFAD2, FANCD, Fanconi anemia complementation group D2, Fanconi anemia group D2 protein, Fanconi anemia, complementation group D2, FLJ23826, FPN1, HFE4, IREG1, Protein FACD2, SLC11A3 |
| Gene ID (Entrez) | 2177 |
| Formulation | PBS with 0.05% Sodium Azide |
| Immunogen | This FANCD2 Antibody was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9] |
| Classification | Polyclonal |
| Primary or Secondary | Primary |
| Test Specificity | This antibody is specific for FANCD2. |