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Buffers and Standards

Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.

The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:

  • Red: pH 4.0
  • Yellow: pH 7.0
  • Blue: pH 10.0

 

Chromatography Standards (199)
Life Science Buffers (115)
Spectroscopy Standards (89)
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Karl Fischer Buffers and Reagents (18)
Electrochemistry Buffers (8)
Matrix Blanks and Standardization Solutions (6)
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Keyword Search:
1 – 15 of 241 results
1

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

EDGE
Physical Form Liquid
Recommended Storage Room temperature
2

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol

EDGE
Molecular Weight (g/mol) 238.30
ChEBI CHEBI:42334
Color White
Chemical Name or Material HEPES
Grade Molecular Biology
SMILES OCCN1CCN(CCS(O)(=O)=O)CC1
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol Gray
Assay Percent Range ≥99 %
PubChem CID 23831
Absorbance 0.01 max. (0.1M solution) at 280nm
Percent Purity ≥99%
CAS 7365-45-9
Protease Protease free
Health Hazard 3 Emergency Overview
Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. .

NFPA

Health:2
Flammability:1
Instability:1
MDL Number MFCD00006158
Health Hazard 2 WARNING!
Solubility Information Soluble in water
pH 5.0 to 6.5
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
3
Promotions Available

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

EDGE
Color Colorless
Physical Form Liquid
Chemical Name or Material Tris-EDTA
Grade Molecular Biology
DNase DNase free
Filtered Through Filtered through a 5-micron filter.
ChemAlert Storage Symbol Gray
Name Note 1X Solution, pH 8.0
CAS 7732-18-5
Protease Protease free
Health Hazard 3 Emergency Overview
May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention.

NFPA

Health:1
Flammability:0
Instability:0
Health Hazard 2 CAUTION!
pH 7.4 to 8.1
Synonym TE
Recommended Storage RT
4

Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

EDGE
PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
SMILES C(C(CO)(CO)N)O
Synonym Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
5

TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™

Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

EDGE
Name Note 50X Solution
Concentration or Composition (by Analyte or Components) Tris (24%), Acetic Acid (5.0%), and EDTA (<2%).
CAS 6850-28-8
Protease Protease free
Physical Form Liquid
Grade Electrophoresis
Synonym TAE
Purity Grade Notes DNase-, RNase- and Protease-Free
DNase DNase free
Recommended Storage RT
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
6
Promotions Available

PBS, Phosphate Buffered Saline, 1X Powder, pH 7.4, Fisher BioReagents

EDGE
Color White
Packaging Poly Bottle
Physical Form Powder
Recommended Storage Room temperature
7

TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

EDGE
Boiling Point 100°C
Color Colorless
Physical Form Liquid
Chemical Name or Material Tris Buffered Saline
Grade Molecular Biology
DNase DNase free
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS 7732-18-5
Protease Protease free
pH 7.5
Synonym TBS
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
8
Promotions Available

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™

Commonly used buffering agent

EDGE
Color Undesignated
Physical Form Liquid
Chemical Name or Material HEPES Buffer
Grade Molecular Biology
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
Concentration 0.95 to 1.05 M
ChemAlert Storage Symbol Gray
Absorbance ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm
Name Note 1M Solution, pH 7.3
CAS 7732-18-5
Protease Protease free
pH 7.2 to 7.5
Synonym N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
Formula Weight 238.197
9

Tris Hydrochloride, 1M Solution (pH 7.5/Mol. Biol.), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

EDGE
PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
SMILES C(C(CO)(CO)N)O
Synonym Tris-HCl
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
10

PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4, Fisher BioReagents™

EDGE
Packaging Poly Cube
Physical Form Liquid
pH 7.3 to 7.5
Recommended Storage Room temperature
Melting Point 0°C
11

Tris-Glycine-SDS, 10X Solution (Electrophoresis), Fisher BioReagents™

EDGE
Color Undesignated
Physical Form Liquid
Chemical Name or Material Tris-Glycine-SDS
Grade Electrophoresis
Identification Pass Test
DNase DNase free
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) 0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS.
CAS 151-21-3
Protease Protease free
Synonym TG-SDS
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
12

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

EDGE
13

Tris Base (White Crystals or Crystalline Powder/Triple-Crystallized), Fisher BioReagents™

For sensitive molecular biology, tissue culture, and electrophoresis methods | CAS: 77-86-1 | C4H11NO3 | 121.136 g/mol

EDGE
14

TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™

CAS: 10043-35-3 Synonym: TBE

EDGE
CAS 10043-35-3
Synonym TBE
15

Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

EDGE
PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
SMILES C(C(CO)(CO)N)O
Synonym Tris-HCl
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3