Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
Synonym | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Tris Hydrochloride, 1M Solution (pH 7.5/Mol. Biol.), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
Synonym | Tris-HCl |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
Chemical Name or Material: Tris-Borate-EDTA Name Note: 10X Solution CAS: 10043-35-3 ChemAlert Storage Symbol: Gray Color: Colorless CAS: 60-00-4 Recommended Storage: RT CAS: 77-86-1 CAS: 7732-18-5 pH: 8.2 to 8.4 Physical Form: Liquid DNase: DNase free Filtered Through: Filtered through a 0.2-micron filter. Purity Grade Notes: DNase-, RNase- and Protease-Free Protease: Protease free Synonym: TBE Grade: Electrophoresis
Color | Colorless |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-Borate-EDTA |
Grade | Electrophoresis |
DNase | DNase free |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 8.2 to 8.4 |
Synonym | TBE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Molecular Weight (g/mol) | 238.30 |
---|---|
ChEBI | CHEBI:42334 |
Color | White |
Chemical Name or Material | HEPES |
Grade | Molecular Biology |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
Identification | Pass Test |
DNase | DNase free |
Merck Index | 15, 4689 |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
ChemAlert Storage Symbol | Gray |
Assay Percent Range | ≥99 % |
PubChem CID | 23831 |
Absorbance | 0.01 max. (0.1M solution) at 280nm |
Percent Purity | ≥99% |
CAS | 7365-45-9 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
MDL Number | MFCD00006158 |
Health Hazard 2 | WARNING! |
Solubility Information | Soluble in water |
pH | 5.0 to 6.5 |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Molecular Formula | C8H18N2O4S |
TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™
Boiling Point | 100°C |
---|---|
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
DNase | DNase free |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.5 |
Synonym | TBS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents
Color | Colorless |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Filtered Through | Filtered through a 5-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 1X Solution, pH 8.0 |
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Health Hazard 2 | CAUTION! |
pH | 7.4 to 8.1 |
Synonym | TE |
Recommended Storage | RT |
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Packaging Type | Poly Cube |
pH | 7.3 to 7.5 |
Melting Point | 0°C |
Tris Hydrochloride (Small White Flakes/Molecular Biology), Fisher BioReagents
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: Tris(hydroxymethyl)aminomethane Hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
PubChem CID | 93573 |
---|---|
CAS | 1185-53-1 |
Molecular Weight (g/mol) | 157.594 |
SMILES | C(C(CO)(CO)N)O.Cl |
Synonym | Tris(hydroxymethyl)aminomethane Hydrochloride |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
Molecular Formula | C4H12ClNO3 |
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
Name Note | 50X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
CAS | 6850-28-8 |
Protease | Protease free |
Physical Form | Liquid |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Fisher BioReagents™ Phosphate Buffered Saline Tablet
Each tablet prepares 100mL of 1X PBS solution. Fisher Bioreagents™ Phosphate Buffered Saline tablets (PBS) are a convenient option for making 1X phosphate buffered saline.
Color | Colorless |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Filtered Through | Filtered through a 5-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 1X Solution, pH 7.4 |
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Health Hazard 2 | CAUTION! |
pH | 7.4 to 8.1 |
Synonym | TE |
Recommended Storage | RT |
Name Note | 100X Solution |
---|---|
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview Irritating to eyes and skin. Wear personal protective equipment. Ensure adequate ventilation. Avoid contact with skin, eyes and clothing. Avoid ingestion and inhalation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Get medical attention immediately if symptoms occur. Do not induce vomiting. NFPA Health:2 Flammability:0 Instability:0 |
Health Hazard 2 | WARNING! |
Color | Undesignated |
Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |