General Western Blot Reagents
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Invitrogen™ Bolt™ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
| Product Type | Transfer Buffer |
|---|
Thermo Scientific™ SuperSignal™ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
| Content And Storage | Upon receipt store solutions at 4°C Products are shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Type | Western Blot Enhancer |
| Membrane Compatibility | Nitrocellulose, PVDF |
| For Use With (Application) | Western Blot |
| Product Line | SuperSignal |
| Detection Method | Chemiluminescence, Fluorescence, Colorimetric |
Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer
Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.
| Content And Storage | Upon receipt store at room temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore |
Invitrogen™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)
Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels
Invitrogen™ iBind™ Flex Fluorescent Detection (FD) Solution Kit
Detection kit for use with iBind™ Flex Western System
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
| Content And Storage | Upon receipt store at room temperature. Product shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore |
| No. of Reactions | 10 midi blots or 20 mini blots |
|---|---|
| Product Type | Flex Solution Kit |
| For Use With (Equipment) | iBind Flex Western Device |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | iBind |
| Detection Method | Colorimetric, Chromogenic, Chemiluminescence |
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
| Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore |
Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer
Cytiva ECL Select Western Blotting Detection Reagent
Highly intense signal output for detection of medium to very low protein levels. Cytiva ECL Select Western Blotting Detection Reagent is a high sensitivity reagent for chemiluminescent Western blotting detection. Includes Luminol and Peroxide solutions, sufficient for 1000cm2 membrane.
Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane
Nitrocellulose membrane, with a 0.45 μm pore size, is suitable for proteins of a wide range of molecular weights.
Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-mouse
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-rabbit
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Invitrogen™ XCell II™ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes