General Western Blot Reagents
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Thermo Scientific™ Pierce™ Western Blot Signal Enhancer
Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
| Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore |
Invitrogen™ Novex™ pH 3-7 IEF Buffer Kit, Includes LC5300, LC5370, LC5371
Optimized for using with Novex pH 3-7 IEF gels
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
| Content And Storage | Upon receipt store at room temperature. Product shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore |
Invitrogen™ Bolt™ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Cytiva ECL Select Western Blotting Detection Reagent
Highly intense signal output for detection of medium to very low protein levels. Cytiva ECL Select Western Blotting Detection Reagent is a high sensitivity reagent for chemiluminescent Western blotting detection. Includes Luminol and Peroxide solutions, sufficient for 1000cm2 membrane.
Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane
Nitrocellulose membrane, with a 0.45 μm pore size, is suitable for proteins of a wide range of molecular weights.
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Invitrogen™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
| Shipping Condition | Dry Ice |
|---|---|
| Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
| Product Type | Protein Labeling Reagent |
| For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Quantity | 1 kit |
| Shelf Life | At least 2 years from receipt |
| Run Time | 10 minutes |
| For Use With (Application) | Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
| Gel Compatibility | Any Gel Type and Gel Chemistry |
| Product Line | Invitrogen |
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
| Product Type | Transfer Buffer |
|---|
Invitrogen™ iBind™ Flex Fluorescent Detection (FD) Solution Kit
Detection kit for use with iBind™ Flex Western System
Invitrogen™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)
Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels
| No. of Reactions | 10 midi blots or 20 mini blots |
|---|---|
| Product Type | Flex Solution Kit |
| For Use With (Equipment) | iBind Flex Western Device |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | iBind |
| Detection Method | Colorimetric, Chromogenic, Chemiluminescence |
Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-rabbit
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)