PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.

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Applied Biosystems™ Region of Interest (ROI) and Background Plates, 96-well

Used to maintain the ViiA™ 7, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with 96-well (0.2 mL) blocks.

Pricing and Availability

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Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Format	Tube
For Use With (Equipment)	QuantStudio™ 6 Pro or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, QuantStudio™ Absolute Q Digital PCR System, ViiA™ 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier
Sample Type	DNA, RNA
Reaction Speed	Fast or Standard
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	1-step RT-qPCR,Multiplex qPCR
Product Line	TaqPath™
Detection Method	Primer-probe

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Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Hot Start	Built-In Hot Start
Product Line	TaqMan™

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Applied Biosystems™ Absolute Q™ DNA Digital PCR Master Mix (5X)

Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	PowerUP™, SYBR™
Detection Method	SYBR

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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Pricing and Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	5 U/μL
Exonuclease Activity	5' - 3'

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Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Pricing and Availability
Specifications

Shipping Condition	Room Temp or Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Overhang	3'-A
Green Features	Sustainable packaging
Concentration	2X
For Use With (Application)	Cloning,Hot-start PCR
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq Gold 360 DNA Polymerase
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	PCR Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Starting Material	DNA

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Applied Biosystems™ Protein Thermal Shift™ Dye Kit

This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

Pricing and Availability

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TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Genotyping Target	Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Product Type	Genotyping Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Sample Type	DNA (Genomic)
Reaction Speed	Standard
Technique	SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Product Line	TaqMan™

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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
Concentration	2X
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	qPCR
Detection Method	SYBR
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudio™ Absolute Q Digital PCR System, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Product Line	Applied Biosystems™

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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25 X
Description	Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase	ArrayScript™
Concentration	25X
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Product Type	One-Step RT-PCR Reagent
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type	RNA
Reaction Speed	Standard
Product Line	AgPath-ID™, Ambion™

Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)

Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ RT-PCR System.

Pricing and Availability

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Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan™

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Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Sufficient For	100 Reactions of 20 μL
Product Line	SYBR™

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Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Pricing and Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Standard
Label or Dye	SYBR Green
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	SYBR™
Detection Method	SYBR

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Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix