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Gene Reporter Systems

Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
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Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

EDGE
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10
Vector TOPO-TA Cloning Vectors
Product Line One Shot
2

Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System

Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.

EDGE
Content And Storage This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase.
Store the product at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate CSPD™
Assay Reporter Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line NovaBright, Phospha-Light
3

Invitrogen™ Yeast tRNA

Supplied in lyophilized form

EDGE
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Content And Storage Store in freezer (-5 to -30°C).
Form Lyophilized Powder
Product Type Yeast tRNA
Reagent Type Yeast tRNA
For Use With (Application) Carrier for Nucleic Acid Precipitation
4

Invitrogen™ TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit (Green Fluorescence), for live-cell imaging

Contains an expression tag-based fluorescence labeling reagent for live-cell labeling

EDGE
5

Invitrogen™ Mouse Cot-1 DNA

Mouse DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the B1, B2, and L1 family members

6

Invitrogen™ MessageAmp™ II aRNA Amplification Kit

Amplifies as little as 100pg of input RNA for GeneChip™ analysis

Format Kit
Reverse Transcriptase ArrayScript™
Includes Label or Dye No
Product Type aRNA Amplification Kit
Sample Type Poly(A+) RNA, Total RNA
Product Line Ambion, MessageAmp
7

Invitrogen™ RadPrime DNA Labeling System

Ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for the detection of DNA and RNA

8

Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Star™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line Gal-Screen, NovaBright
9

Invitrogen™ Light Emission Accelerator-II

Light emission triggering solution with luminescence enhancer for use with the Galacto-Light Plus™ or Dual-Light™ Reporter Gene Assay Systems.

10

Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection

Chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase or the non-secreted placental isozyme PLAP.

Substrate CSPD™
Substrate Properties Chemical Substrate
Content And Storage Component solution containing CSPD™ substrate and chemiluminescence enhancer (Store at 2°C to 6°C), placental alkaline phosphatase positive control (Store at ≤ -20°C)
Format 96-well Plate
For Use With (Equipment) Luminometer (Microplate)
Product Type Phosphatase Assay
Target Enzyme Placental Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
High-throughput Compatibility High-throughput Compatible
Product Line NovaBright
Detection Method Enhanced Chemiluminescence
12

Invitrogen™ CytoTune™ EmGFP Sendai Fluorescence Reporter

The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS Sendai reprogramming kits can transduce into a given cell type before you advance to one of these kits.

13

Invitrogen™ ArrayControl™ RNA Spikes

Add to the RNA sample for use as a template for preparing labeled cDNA

14

Invitrogen™ Human Cot-1 DNA™-Fluorometric QC

Quantifying by fluorometry provides greater data accuracy

15

Invitrogen™ Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Rapid, ultrasensitive detection of β-galactosidase in reporter gene assays. Thermo Scientific Galacto-Light Plus β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.

Content And Storage This kit contains the lysis solution, substrate, reaction buffer, and accelerator.
Store at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Plus™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated