Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ FastDigest BamHI
Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | BamH I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 80°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest PaeI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nco I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest SacI
The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SacI |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 3' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest NotI
The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Not I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 80°C |
| Product Line | FastDigest |
Thermo Scientific™ AarI (2 U/μL)
Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | AarI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer AarI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 2 U/μL |
Thermo Scientific™ FastDigest AatII
Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).
Thermo Scientific™ DpnI (10 U/μL)
Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ FastDigest SmiI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest PagI
Cut at T^CATGA sites with Thermo Scientific™ FastDigest™ BspHI (PagI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciI, RcaI).
Thermo Scientific™ FastDigest ScaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Eco81I (Bsu36I) (10 U/μL)
The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco81I (Bsu36I) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest DraI
Cut at TTT^AAA sites with Thermo Scientific™ FastDigest™ DraI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest SdaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest XagI
Cut at CCTNN^NNNAGG sites with Thermo Scientific™ FastDigest™ EcoNI (XagI), which performs best using universal FastDigest Buffer (Isoschizomers: BstENI).