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1

Cytiva TempliPhi™ 2000 Reaction Kit

Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications

2

Cytiva TempliPhi™ 100/500 Amplification Kit

Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.

Includes 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA
Content And Storage The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification
Description illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
For Use With (Application) Sequencing Template Amplification Kits and Reagents
Test Time 4-18 h depending on starting material
3

Cytiva TempliPhi™ Sequence Resolver Kit
GREENER_CHOICE

Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates

Content And Storage Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
Description illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
Product Type illustra TempliPhi Sequence Resolver Kit
For Use With (Application) Amplification of difficult templates for successful DNA sequencing
Test Time 18 h