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Cytiva HiTrap™ Desalting Prepacked Columns
For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances.
Disposable | No |
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Particle Size | >20 μm (dry), 15 to 88 μm (wet) |
Content And Storage | 4°C to 30°C in 20% Ethanol |
Diameter (English) Inner Column | 0.62 in. |
Description | HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange. |
Volume (Metric) Bed | 5 mL |
For Use With (Application) | For rapid convenient desalting and buffer exchange |
Diameter (Metric) Inner Column | 16 mm |
Cytiva HiTrap™ Capto™ Adhere Columns
Designed to remove contaminants in post-protein A purification
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva HiTrap IXSelect
HiTrap IXSelect columns are prepacked with IXSelect resin for screening and small-scale protein purifications using affinity chromatography.
Content And Storage | 2°C to 8°C |
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Cytiva HiTrap Capto Butyl
Capto Phenyl (High sub) and Capto Butyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification
Height (Metric) | 25 mm |
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Flow Rate | < 600 cm/h |
Storage Requirements | 4 to 30 °C, 20% Ethanol |
For Use With (Application) | Hydrophobic Interaction Chromatography |
Cytiva HiTrap™ GCSFSelect
Prepacked columns specifically designed for the purification of recombinant Granulocyte-colony stimulating factor (G-CSF). Based on a highly rigid agarose base matrix that allows for high flow rates at large production scales.
Particle Size | 75 μm |
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Ligand Type | Single-Chain Antibody Fragment Directed Against G-CSF |
Type | Column |
For Use With | Purification of Recombinant Granulocyte-Colony Stimulating Factor (G-CSF) |
pH | 2 to 12 |
Quantity | 5/Pk. |
Purification Target | Granulocyte-Colony Stimulating Factor (G-CSF) |
Cytiva HiTrap™ Heparin HP Columns
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
Particle Size | 34 μm |
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Ligand Type | 10mg Heparin/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 30°C |
pH Range | 5 to 10 |
Product Type | Heparin HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Sample Type | DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs |
Material | Polypropylene |
For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Butyl HP Columns
Prepacked columns for convenient and fast separations. Cytiva HiTrap™ Butyl HP Columns are used for small-scale protein purification as well as for screening of binding and elution conditions.
Particle Size | 24 to 44 μm |
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Ligand Type | Butyl |
Height (Metric) | 30 mm |
pH Range | 3 to 13 |
Product Type | HiTrap Butyl HP |
Material | Polypropylene |
Storage Requirements | 4°C to 30°C, 20% Ethanol |
For Use With (Application) | For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications |
Support Type | Cross-linked Agarose |
Media Type | Butyl Sepharose |
Max. Pressure | 5 bar [0.5 MPa] (70 psi) |
Height (English) | 1.181 in. |
Cytiva HiTrap Capto MMC Columns
HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 2 to 12 |
Product Type | HiTrap Capto MMC column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Bind Proteins at the conductivity of the feed material and to solve specific purification challenges |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Streptavidin HP Column
Use for fast and easy preparative purifications either alone or connected in series. HiTrap™ Streptavidin HP Column are convenient and reliable prepacked columns.
Cytiva HiTrap™ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.
Particle Size | 34 μm |
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Ligand Type | 10μM NHS groups/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 8°C |
pH Range | 3 to 12 |
Product Type | NHS-Activated HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Material | Polypropylene |
For Use With (Application) | Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Column Type | Column Accesory |
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Material | Polypropylene |
Cytiva HiTrap™ Protein L Prepacked Columns
For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs
Particle Size | 85μm |
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Ligand Type | Recombinant Protein L (E. coli), mammalian free |
Flow Rate | <500cm/hr. |
Height (Metric) | 20mm |
Content And Storage | 2° to 8°C |
pH Range | 2 to 10 |
Product Type | HiTrap Protein L column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Affinity chromato graphy medium (Resin) for capture of Antibodies and Antibody fragments such as Fab fragments, scFv and Dabs |
Support Type | Highly Cross-linked Agarose |
Max. Pressure | 5 bar |
Height (English) | 0.78 in. |
Cytiva HiTrap Capto Phenyl ImpRes
HiTrap Capto Phenyl ImpRes is prepacked with BioProcess Capto Phenyl ImpRes resins for screening and small-scale protein purifications using hydrophobic interaction chromatography.
Cytiva HiTrap Capto Lentil Lectin
Capto Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues.