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Thermo Scientific™ Buffer Set for Restriction Enzymes
Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.
Thermo Scientific™ TheraPure™ Restriction Enzymes
Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.
| Cut Site | CTCTTC(1/4)^ |
|---|---|
| Content And Storage | Store at -20°C. |
| Form | Liquid |
| Enzyme | Eam1104I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Quantity | 3000 U, 150 μL |
| Type IIS RE | Yes |
| Incubator Temperature | 37°C |
| Concentration | 20 U/μL |
| For Use With (Application) | In vitro Transcription |
| Inactivation Temperature | 65°C |
| Sensitive to Heat Inactivation | Yes |
| Product Type | Restriction Enzyme |
| Research Category | Drug Development |
| Compatible Buffer | 10X Tango Buffer |
| Optimal Reaction Temperature | 37°C |
| Product Line | TheraPure™ |
Thermo Scientific™ Dilution Buffer for Restriction Enzymes
Optimize applications that require diluted enzymes with this dilution buffer.
| Shipping Condition | Dry Ice |
|---|---|
| Product Type | RNAse Cocktail Enzyme Mix |
| Enzyme | RNase |
| Product Line | Ambion™ |
1250 UNIT RESTRICTION ENZYME SCA I STORE AT -20°C
10368352 1250 UNIT RESTRICTION ENZYME SCA I STORE AT -20°C
Invitrogen™ Gateway™ LR Clonase™ II Enzyme mix
Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | Gateway™ LR Clonase™ II enzyme mix includes proteinase K solution (2 μg/μL) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored. |
| Product Type | LR Clonase Enzyme Mix |
| Enzyme | LR Clonase |
| Compatible Buffer | Enzyme Buffer |
| Product Line | Clonase™, Gateway™ |
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
| Format | Kit |
| Sample Type | PCR Products |
| Isolation Technology | Silica Spin Column |
| Run Time | 10 min. |
Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
|---|---|
| Format | Kit |
| Sample Type | PCR Products |
| Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
| For Use With (Application) | PCR Cloning |
| Vector | pJET1.2/blunt |
| Product Line | CloneJET |
Thermo Scientific™ pBR322 Plasmid DNA
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
Thermo Scientific™ HincII (HindII) (10 U/μL)
The HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: HindII).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | HincII (HindII) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ PscI (PciI) (10 U/μL)
The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PscI (PciI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ TaqI (10 U/μL)
The TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65°C in its own unique buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ Eco81I (Bsu36I) (10 U/μL)
The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco81I (Bsu36I) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ Hin6I (HinP1I) (10 U/μL)
Cut at G^CGC sites with Hin6I (HinP1I) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AspLEI, BstHHI, CfoI, HinP1I, HspAI).
Thermo Scientific™ PfeI (TfiI) (10 U/μL)
Cut at G^AWTC sites with PfeI (TfiI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: TfiI).