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Invitrogen™ Retic Lysate IVT™ Kit

Efficiently translates high molecular weight proteins and both capped and uncapped mRNAs

Brand:  Invitrogen™ AM1200M

Additional Details : Weight : 0.04000kg

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Product Code. 10005314

  • 359.00 € / Each
In stock, ready to ship: 4

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Description

Description

The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimized lysate results in high protein yield, biological activity, and consistent performance. A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. The Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG.

  • Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs
  • Accommodates large volume of template mRNA
How it works
The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA. Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.

Specifications

Specifications

In Vitro Transcription
60 reactions
Cell-Free
Water Soluble Protein
Kit with manual
Cell-Free Expression
Kit
Ambion™
Videos
SDS
Documents
Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.