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Invitrogen™ ULTRAhyb™ Ultrasensitive Hybridization Buffer
Proprietary blend of hybridization accelerators and blocking agents that greatly enhance the levels of hybridization
281.00 € - 908.00 €
Specifications
| Content And Storage | Store at 4°C. |
|---|---|
| Buffer | Hybridization Buffer |
| Product Type | Ultrasensitive Hybridization Buffer |
| Shipping Condition | Room Temperature |
Description
- Signals that once took days to visualize are now apparent in hours
- It is supplied in four bottles containing 125mL each
- Increase the sensitivity of blot hybridization experiments
- Increase random-primed DNA probe sensitivity up to 100x; RNA probe sensitivity 20x
- Decrease hybridization time to 2 hours
- Using standard hybridization buffers, only 1 to 5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19); With ULTRAhyb Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected
- Compatible with RNA and DNA probes labeled isotopically and nonisotopically in northerns, Southerns, and dot/slot blots
- Contains 50% formamide and is compatible with positively charged membranes
- Requires 10-100x less nonisotopic DNA probe than standard hybridization buffers, making it possible to perform many more hybridizations with a single labeling reaction
Applications:
DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting
Order Info
Shipping Condition: Room Temperature
Specifications
| Store at 4°C. | |
| Hybridization Buffer | |
| Ultrasensitive Hybridization Buffer | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.