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BrdU Mouse, Alexa Fluor 555, Clone: 3D4, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 560210

Additional Details : Weight : 0.01000kg

Product Code. 15830019

  • 770.00 € / Each
Estimated Shipment: 22-04-2024
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Description

Description

Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the cells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics.

The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU.

Specifications

Specifications

BrdU
Monoclonal
Alexa Fluor 555
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Affinity Purified
RUO
Primary
IgG1 κ
In vitro Assay
3D4
5-bromo-2'-deoxyuridine, 5-Bromouracil deoxyriboside, BUdR
Mouse
100 Tests
Cell Biology
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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