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Cytiva TempliPhi™ Sequence Resolver Kit

Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates

464.00 € - 2384.00 €

Specifications

Content And Storage Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
Description illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
For Use With (Application) Amplification of difficult templates for successful DNA sequencing
Product Type illustra TempliPhi Sequence Resolver Kit
Test Time 18 h
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Products 2
Product Code Brand Includes No. of Reactions Price Quantity & Availability  
Product Code Brand Includes No. of Reactions Price Quantity & Availability  
10308259
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Cytiva
28-9035-31
1 x 900μL Sample Buffer, 1 x 900μL Reaction Buffer, 1 x 100μL Enzyme mix 200
2384.00 €
1 set
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Estimated Shipment: 14-05-2024
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11339941
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Cytiva
28-9035-29
1 x 90μL Sample Buffer, 1 x 90μL Reaction Buffer, 1 x 90μL Enzyme mix 20
464.00 €
1 set
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Estimated Shipment: 14-05-2024
to see stock.
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Description

Description

  • Cultureless preparation from bacterial colonies, glycerol stock or purified DNA, saves time and reagents
  • Requires 20 minutes hands-on time
  • Excellent sequence finishing performance resolving sequencing stop and compression issues associated with difficult templates
  • Improve difficult template sequencing success with up to 820 bp Phred 20 read length
  • For use directly in cycle sequencing reactions without purification
  • Available in sizes of 20, 50 and 500 reactions

Specifications

Specifications

Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
Amplification of difficult templates for successful DNA sequencing
18 h
illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
illustra TempliPhi Sequence Resolver Kit
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