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Invitrogen™ IFN beta Human ELISA Kit
Measure levels of human interferon beta (IFNb) in human cell and tissue culture media supernatant samples using 96-well plates.
Brand: Invitrogen™ 414102
Code : A0
Additional Details : Weight : 0.10000kg
Description
Assay IFN-b (human interferon beta) levels in human cell and tissue culture media (supernatants) with theseInvitrgen™ Human IFN beta ELISA Kits.
These human IFN beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon beta (IFN-β) in samples at 100μL per well. These IFN-β kits are validated for use with human cell and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.Target: Human interferon beta (human IFN-β, IFN-beta)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well plate
Measurement: Absorbance at 450nm
Assay Range: 25 to 2000pg/mL
Sensitivity: Undetermined
Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: Cell culture supernatant, tissue culture media
Total Assay Time: 3.25 hours
Sample Size: 100μL
Specificity: No significant cross-reaction with human IFN-α or IFN-γ; mouse or rat IFN-β
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.
Specifications
| 25 to 2000pg/mL | |
| Human IFN beta ELISA Kit | |
| Cell Culture Supernatant, Tissue Culture Media | |
| 5 x 96 microplate wells | |
| Absorbance at 450nm | |
| 5 Plate Kit | |
| 3.25 hr. |
| Undetermined | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with human IFN-a or IFN-g; mouse or rat IFN-ß | |
| Human IFN Beta Standard 100,000pg/mL, 15 vials, HRP Conjugate Concentrate, 1 vial, Concentrate Diluent, 150mL, Antibody Concentrate, 1 vial, Precoated 96-well Plates, 5 ea, Sample Diluent, 150mL, Stop Solution, 60mL, TMB Substrate Solution, 60mL, Wash Solution Concentrate, 250mL, Plate Sealers, 20 ea | |
| Intra-assay CV ≤ 10%; inter-assay CV ≤ 10% | |
| Human interferon beta (human IFN-ß, IFN-beta) |