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Macherey-Nagel Bioanalysis™ NucleoSpin™ 96 Blood QuickPure, 96-Well Kit
Ultra-fast isolation of highly concentrated DNA from blood in proven 96‐well plate format.
Brand: Macherey-Nagel Bioanalysis™ 740667.24
This item is not returnable.
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Description
NucleoSpin™ Blood QuickPure binding plates are to be used in a centrifuge with deep buckets allowing processing of NucleoSpin™ 96 well plates stacked on 96 well blocks. The method is based on a specially treated silica membrane which speeds up the procedure by a combined washing/drying step. The number of washing and drying steps is reduced from 3 to 1. Therefore the hands-on time is minimised. Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of proteinase K. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin™ Blood QuickPure binding strips are created by addition of ethanol to the lysate. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and can be used directly for PCR, Southern blotting or any kind of enzymatic reaction. The kit allows isolation of 4 μg to 6 μg of pure genomic DNA from 200 μL whole blood with an A260/280 ratio between 1.60 and 1.90 and a typical concentration of 40 to 60 ng/μL.Kit components: NucleoSpin™ Blood QuickPure binding plates, buffers, proteinase K, accessories.
- Silica membrane technology
- Manual processing
- Yield: 4 to 6 μg
- Fragment size: 300 bp to 30 kb
- Sample material: human blood or other body fluid (200 μL/300 μL), 107 lymphocytes
- Elution volume: 75 to 100 μL
- Binding capacity: 60 μg
- Preparation time: 30 min/prep
Specifications
| 18°C to 25°C | |
| Isolation of DNA | |
| DNA |
| 96-well Plate | |
| 2304 Preps |
Safety and Handling
Hazard Category
- Skin sensitiser Category 1