OPTIZYME™ NotI, Fisher BioReagents™
Brand: Fisher Bioreagents BP8004-5
Code : 32
Additional Details : Weight : 0.01000kg
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Conditions for 100% Activity
- 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 , 100mM NaCl and 0.1mg/ml BSA.
- Incubate at 37°C.
Enzyme Activity in OPTIZYME buffers
- Buffer 1: 0 - 20%
- Buffer 2: 20 - 50%
- Buffer 3: 100%
- Buffer 4: 0 - 20%
- Buffer 5: 20 - 50%
20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Bsp120I, CfrI, Eco52I.
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: completely overlaps - blocked.
|25 to 50% Water, >50% Glycerin|
|20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol|
|>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I|
|Keep container tightly closed|