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Molecular Probes™ pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate

pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate

Brand:  Molecular Probes™ P35374

Additional Details : Weight : 0.00910kg

Product Code. 15306794

  • 538.00 € / 20µg
Estimated Shipment: 13-05-2024
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Description

Description

pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo™ Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

  • pHrodo™ Red dye conjugated directly to highly active human recombinant EGF molecule
  • Excitation/emission of 560/585 nm
  • Excellent photostability
  • Appropriate for multiplexing with green fluorophores such as GFP, FITC, and Alexa Fluor™ 488

    pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos™, FLoid™, Tali™, and Attune™ instruments.
  • Specifications

    Specifications

    Orange
    pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate, 20μg, Orange, Lyophilized, 560/585nm Excitation/Emission, pH-Sensitive Dyes, Used with Flow Cytometer, Fluorescence Microscope, Fluorometer, High Content Instrument
    Fluorescence Microscopy, High-content Screening (HCS), High-throughput Screening (HTS), Flow Cytometry
    pHrodo™ Red Epidermal Growth Factor Conjugate
    560nm
    –5 to –30°C
    Fluorescent
    Flow Cytometer, Fluorescence Microscope, Fluorometer, High Content Analysis Instrument
    pH-Sensitive dyes
    20μg
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    For Research Use Only. Not for use in diagnostic procedures.