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Macherey-Nagel™ Plasmid DNA purification, NucleoSpin™ , Neutralization Buffer A3

Brand:  Macherey-Nagel™ 740913.1

Code : LE

Additional Details : CAS Number : 50-01-1 Weight : 1.14000kg


Product Code. 12776553

  • 174.00 € / 1000mL
Estimated Shipment: 10-06-2024
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Description

Description

The eight-well format of the NucleoSpin™ miniprep columns is easily processed by vacuum filtration. The NucleoVac 96 vacuum manifold allows simultaneous processing of multiples of eight samples in less than 90min. The NucleoSpin™ Multi-8 Plus Plasmid kit also contains NucleoSpin™ Plasmid filter strips to clear bacterial lysates instead of time consuming centrifugation. The NucleoSpin™ Multi-8 Plus Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mLE. coliovernight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt. After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used. Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, buffers, RNase A, MN wash plate, MN tube strips, cap strips. For further information on this product contact your local Customer Services.
  • Silica-membrane technology
  • Yield: up to 20μL sequencing grade plasmid DNA
  • Elution volume: 75μL to 150μL
  • Culture volume: 1mL to 5mL
  • Binding capacity: 25μg
  • Maximum plasmid size <15kb
  • No use of organic solvents
  • NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included Rapid small-scale preparation of high-copy plasmids fromE. coli.
Specifications

Specifications

1000 mL
For Plasmid DNA Purification
Neutralization Buffer A3
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Safety and Handling

Safety and Handling

Product Identifier
  • Buffer A3 (1000 mL)

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.

Supplemental information
  • MIXTURE LIST-Contains : guanidium hydrochloride

SDS
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Special Offers

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