Buffers and Standards
Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.
The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:
- Red: pH 4.0
- Yellow: pH 7.0
- Blue: pH 10.0
Filtered Search Results
Buffer Solution, pH 10.00, Reagecon™
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
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Supports traceability with guaranteed access to certificates and proactive change notifications.
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Certified by an ISO 17025 accredited test method and fully NIST traceable. Reagecon™ Buffer Solution, pH 10.00 is tested at 20°C or 25°C to an accuracy level of ±0.01. This calibration and control buffer is specially formulated to ensure its stability, even after opening the bottle.
| Boiling Point | 100°C |
|---|---|
| Color | Colorless |
| Solubility Information | Fully miscible in water |
| Traceability to NIST | Yes |
| Physical Form | Liquid |
| Odor | Characteristic |
| Solution Type | pH Buffer |
| Melting Point | 0°C |
Gibco™ DPBS, no calcium, no magnesium
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DPBS, no calcium, no magnesium
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | DPBS |
| Recommended Storage | 15°to 30°C |
| Concentration | 1 X |
Thermo Scientific™ Orion™ pH Buffer Bottles
Perform accurate, reproducible pH calibrations with these color-coded buffer bottles.
| For Use With (Equipment) | pH Meter and Electrode |
|---|---|
| Traceability to NIST | Yes |
| Physical Form | Liquid |
| Chemical Name or Material | pH Buffer, bottles |
pH Buffers, Twin Neck, Fisherbrand™
Perform accurate pH calibrations with color-coded, twin neck Fisherbrand™ pH Buffer Bottles.
| For Use With (Application) | pH calibration |
|---|
Buffer Solution pH4.00 (Phthalate), NIST Standard Solution ready to use for pH measurement, Fisher Chemical™
MDL Number: 217672
| MDL Number | 217672 |
|---|
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Iodine Solution 0.05M (0.1N), NIST Standard Solution Ready To Use, for Volumetric Analysis, meets analytical specification of Ph.Eur., BP, Fisher Chemical™
CAS: 7553-56-2 Molecular Formula: I2 Molecular Weight (g/mol): 253.81 MDL Number: MFCD00011355 MFCD00164163 InChI Key: PNDPGZBMCMUPRI-UHFFFAOYSA-N Synonym: iodine,diiodine,iodine crystals,iodine sublimed,tincture iodine,vistarin,eranol,iodio,iodine solution,iode PubChem CID: 807 ChEBI: CHEBI:17606 IUPAC Name: molecular iodine SMILES: II
| PubChem CID | 807 |
|---|---|
| CAS | 7553-56-2 |
| Molecular Weight (g/mol) | 253.81 |
| ChEBI | CHEBI:17606 |
| MDL Number | MFCD00011355 MFCD00164163 |
| SMILES | II |
| Synonym | iodine,diiodine,iodine crystals,iodine sublimed,tincture iodine,vistarin,eranol,iodio,iodine solution,iode |
| IUPAC Name | molecular iodine |
| InChI Key | PNDPGZBMCMUPRI-UHFFFAOYSA-N |
| Molecular Formula | I2 |
Gibco™ DPBS, calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| pH | 7.0 to 7.2 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| Concentration | 1 X |
| For Use With (Application) | Washing Cells Before Dissociation, Transporting Cells or Tissue Samples, Diluting Cells for Counting, and Preparing Reagents |
| Sterility | Sterile-filtered |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
| Product Line | Gibco™ |
Buffer Colour Coded Solution pH10.00 (Borate) Blue, NIST Standard Solution ready to use for pH measurement, Fisher Chemical™
CAS: 10043-35-3 Molecular Formula: BH3O3 Molecular Weight (g/mol): 61.83 InChI Key: KGBXLFKZBHKPEV-UHFFFAOYSA-N IUPAC Name: boric acid SMILES: OB(O)O
| CAS | 10043-35-3 |
|---|---|
| Molecular Weight (g/mol) | 61.83 |
| SMILES | OB(O)O |
| IUPAC Name | boric acid |
| InChI Key | KGBXLFKZBHKPEV-UHFFFAOYSA-N |
| Molecular Formula | BH3O3 |
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
| Physical Form | Liquid |
|---|---|
| Recommended Storage | Room temperature |
Thermo Scientific™ Orion™ pH Buffer Individual Use Pouches
Ensure fresh, ready-to-use standards for pH calibration with these pH Buffer individual use pouches.
| Boiling Point | 100°C |
|---|---|
| For Use With (Equipment) | pH Meter and Electrode, single-use |
| Solubility Information | Soluble |
| Traceability to NIST | Yes |
| Packaging | Pouch |
| Physical Form | Liquid |
| Chemical Name or Material | pH Buffer, individual use pouches |
| For Use With (Application) | pH calibration |
| Solution Type | Buffer |
Traceable™ ISO 17034 (formerly ISO Guide 34) Color-Coded CRM pH Buffers (Certified Reference Material), Fisherbrand™
Performs accurate, reproducible pH calibrations with color-coded bottles. Fisherbrand™ Traceable, Color-Coded buffers are Certified Reference Material pH Buffers.Compatible with all makes of pH instruments and electrodes.
| For Use With (Equipment) | pH Instruments and Electrodes |
|---|---|
| Physical Form | Liquid |
| Quantity | 473 mL |
| For Use With (Application) | Calibration |
| Solution Type | Buffer |
| Color | Colorless |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-EDTA |
| Grade | Molecular Biology |
| DNase | DNase free |
| Filtered Through | Filtered through a 5-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 1X Solution, pH 8.0 |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| Health Hazard 2 | CAUTION! |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| Recommended Storage | RT |
Thermo Scientific™ Eutech Handheld Meters Calibration Solution, 1413μS/cm KCl
Periodic calibration with accurate standards is recommended for meters
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
| Molecular Weight (g/mol) | 238.30 |
|---|---|
| ChEBI | CHEBI:42334 |
| Color | White |
| Chemical Name or Material | HEPES |
| Grade | Molecular Biology |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| Identification | Pass Test |
| DNase | DNase free |
| Merck Index | 15, 4689 |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| ChemAlert Storage Symbol | Gray |
| Assay Percent Range | ≥99 % |
| PubChem CID | 23831 |
| Absorbance | 0.01 max. (0.1M solution) at 280nm |
| Percent Purity | ≥99% |
| CAS | 7365-45-9 |
| Protease | Protease free |
| Health Hazard 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
| MDL Number | MFCD00006158 |
| Health Hazard 2 | WARNING! |
| Solubility Information | Soluble in water |
| pH | 5.0 to 6.5 |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| Recommended Storage | RT |
| Molecular Formula | C8H18N2O4S |