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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (13,868)
ELISA Substrates and Reagents (1,223)
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1 – 15 of 13,238 results
1
Promotions Available

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2
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Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
3
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Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

EDGE
Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal™
Detection Method Chemiluminescence
4
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Invitrogen™ Human Tau (Total) ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 6.5%
Intraassay CV 4.6
Incubation Time 4 hr.
Gene Symbol DDPAC,FTDP-17 L,MSTD,MTBT1,MTBT2,PPND,PPP1R103,TAU,MAPT
Total Assay Time 4 hrs
Sample Volume Cerebrospinal Fluid 50 μL, Supernatant 50 μL
Assay Sensitivity <10 pg/mL
Storage Requirements 2°C to 8°C
Concentration 31 to 2000 pg/mL
Assay Range 31 to 2000 pg/mL
Marker Gene Tau
Test Time 1 hrs 20 mins
Accession Number P10636
Regulatory Status RUO
Target Tau
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 4137
Sample Type Cerebrospinal Fluid,Supernatant
Label or Dye HRP
5

Invitrogen™ Human IL-8 ELISA Development Kit (ABTS), PeproTech®

ELISA

EDGE
6
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Invitrogen™ Human IFN gamma ELISA Kit

ELISA

EDGE
Measurement Absorbance at 450 nm minus absorbance (at 550 nm)
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Target Species Human
Conjugate HRP
Description Human IFN gamma ELISA Kit
Interassay CV <12%
Intraassay CV <10
Gene Symbol IFG,IFI
Total Assay Time 3 hrs
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity <2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 25.6 to 1000 pg/mL
Test Time 1 hrs 20 mins
Accession Number P01579
Regulatory Status RUO
Target IFN gamma
Specificity No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3458
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
7
Promotions Available

Invitrogen™ Human IL-6 ELISA Kit, High Sensitivity

ELISA

EDGE
8

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

EDGE
9
Promotions Available

Invitrogen™ Human CRP ELISA Kit

ELISA

EDGE
10
Promotions Available

Invitrogen™ Human IL-8 ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 6.1%
Intraassay CV 3.9
Gene Symbol GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Total Assay Time 2 hrs 30 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity <5 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 15.6 to 1000 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hrs 20 mins
Accession Number P10145
Regulatory Status RUO
Target IL-8
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision <5 pg/mL
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3576
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
11
Promotions Available

Invitrogen™ Human Tau (Phospho) [pT181] ELISA Kit

ELISA

EDGE
12

Invitrogen™ Human MMP-12 ELISA Kit

ELISA

EDGE
Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol Mmp12
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.04 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.04 to 10 ng/mL
Test Time 1 hrs 20 mins
Accession Number 3.4.24.65; AV378681; HME; Macrophage elastase; Macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); matrix metalloproteinase-12; ME; MGC138506; Mme; Mmel; MMP; MMP12; MMP-12; MMPs
Regulatory Status RUO
Target MMP12
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 4321
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
13
Promotions Available

Invitrogen™ Human MMP2 ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 5.6%
Intraassay CV 3.3
Incubation Time 4 hr.
Gene Symbol CLG4,CLG4A,MMP-2,MMP-II,MONA,TBE-1
Total Assay Time 4 hrs
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity <1 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.78 to 50 ng/mL
Marker Gene MMP-2
For Use With (Application) Colorimetric Detection
Test Time 1 hrs 20 mins
Accession Number P08253
Regulatory Status RUO
Target MMP2
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 4313
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
14

Invitrogen™ Human LBP ELISA Kit

ELISA

EDGE
Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol LBP
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.82 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.82 to 200 ng/mL
Test Time 1 hrs 20 mins
Accession Number acute phase protein; binds bacterial LPS; BPI fold containing family D, member 2; Bpifd2; hypothetical protein LOC512242; Lbp; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; lipopolysaccharide-binding protein LPSBP; LPS-binding protein; LPSBP; Ly88
Regulatory Status RUO
Target LBP
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3929
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
15

Invitrogen™ Cytokine 30-Plex Human Panel

Specifically designed for quantifying human cytokines, chemokines and growth factors in serum, plasma, and tissue culture supernatant

EDGE