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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (19,567)
ELISA Substrates and Reagents (1,261)
Singleplex and Multiplex Bead Based Immunoassays (1,211)

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1
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Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2

Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

EDGE
Shipping Condition Ambient
Content And Storage Room Temperature
Form Liquid
Product Type ELISA Stop Solution
Substrate Type HRP, TMB
3
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Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

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4
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Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.

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5
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Invitrogen™ Human Cortisol Competitive ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Description Cortisol Competitive ELISA Kit
Interassay CV 8.1%
Intraassay CV 8.8%
Total Assay Time 1 hr 30 min
Sample Volume Fecal Extract 50 μL, Plasma 1 μL, Saliva 12.5 μL, Serum 1 μL, Supernatant 50 μL, Urine 6.25 μL
Assay Sensitivity 17.3 pg/mL
Storage Requirements -20°C
Research Discipline Inflammation
Assay Range 100-3200 pg/mL
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time 1 hr 20 min
Detection Method Fluorescence
Regulatory Status RUO
Target Cortisol
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Sample Type Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine
Label or Dye HRP
6
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Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

EDGE
Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
7

Invitrogen™ Glutathione Colorimetric Detection Kit

Assay Glutathione levels in Whole Blood, Serum, Plasma, Erythrocytes, Urine, Cell Lysates and Tissue Samples (colorimetric).

EDGE
Shipping Condition Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format Colorimetric Detection Kit
Content And Storage 96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet.

Store at –20°C.
For Use With (Equipment) Microplate Reader
Product Type Glutathione Colorimetric Detection Kit
Gene ID (Entrez) Non-Gene
Sample Type Other Biological Fluids,Plasma,Serum
Gene Symbol Non-Gene
Target Kit Named Glutathione
Detection Method Colorimetric
8
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Invitrogen™ ELISA Buffer Kit

Concentrated

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9
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Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

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10
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Invitrogen™ Tissue Extraction Reagent I

Lysis buffer for total protein extraction from tissue samples

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11
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Invitrogen™ Malondialdehyde (MDA) Colorimetric Assay Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

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12
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Invitrogen™ Human IL-6 ProQuantum Immunoassay Kit

ELISA

EDGE
Target Species Human
Conjugate FAM
Gene Symbol Il6
Total Assay Time 2 hr
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.05 pg/mL
Storage Requirements -20°C
Assay Range 0.064-10000 pg/mL
For Use With (Application) Cytokine Protein Quantitation
Test Time 30 min
Detection Method Taqman™ Real-time PCR
Accession Number P05231
Regulatory Status RUO
Target IL-6
Kit Contents Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 3569
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
13

Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use)

The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.

EDGE
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Product Line CDP-Star, NovaBright
14
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Invitrogen™ Human VWF ELISA Kit

ELISA

EDGE
Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10%
Total Assay Time 4 hr 45 min
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Assay Sensitivity 0.13 ng/mL
Research Discipline Angiogenesis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr 20 min
Assay Solid-phase sandwich ELISA
Target VWF
Specificity This ELISA antibody pair detects human vWF. Other species not determined.
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Label or Dye Biotin
Calibration Range 0.4 ng/mL to 120 ng/mL
Interassay CV <12%
Gene Symbol VWF
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 0.123-30 ng/mL
Accession Number P04275
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 7450
Sample Type Plasma,Serum,Supernatant
Synonym F8VWF, VWD
15
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Invitrogen™ Human Tau (Total) ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 6.5%
Intraassay CV 4.6%
Incubation Time 4 hr.
Gene Symbol MAPT
Total Assay Time 4 hr
Sample Volume Cerebrospinal Fluid 50 μL, Supernatant 50 μL
Assay Sensitivity <10 pg/mL
Storage Requirements 2-8°C
Concentration 31 to 2000 pg/mL
Assay Range 31-2000 pg/mL
Marker Gene Tau
Test Time 1 hr 20 min
Accession Number P10636
Regulatory Status RUO
Target Tau
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 4137
Sample Type Cerebrospinal Fluid,Supernatant
Label or Dye Biotin