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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (13,870)
ELISA Substrates and Reagents (1,223)
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1 – 15 of 13,244 results
1

Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA

Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA is designed to detect possible leached ligand of adeno-associated viruses (AAV) purified using POROS CaptureSelect AAVX Affinity Matrix.

EDGE
Shipping Condition Dry Ice
Product Line CaptureSelect
2
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Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

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Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
3

Invitrogen™ Human IL-8 ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 6.1%
Intraassay CV 3.9
Gene Symbol GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Total Assay Time 2 hrs 30 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity <5 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 15.6 to 1000 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hrs 20 mins
Accession Number P10145
Regulatory Status RUO
Target IL-8
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision <5 pg/mL
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3576
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
4
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Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

EDGE
5
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Invitrogen™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs

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6

Invitrogen™ Human IL-6 ELISA Kit

ELISA

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7

Invitrogen™ Human Amyloid beta 42 ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 4.3%
Intraassay CV 3.6
Incubation Time 4 hr.
Gene Symbol AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP
Total Assay Time 4 hrs
Sample Volume Cerebrospinal Fluid 50 μL, Supernatant 50 μL, Tissue Homogenate 50 μL
Assay Sensitivity <10 pg/mL
Storage Requirements 2°C to 8°C
Concentration 15.6 to 1000 pg/mL
Assay Range 15.6 to 1000 pg/mL
Marker Gene Aβ 42
Test Time 1 hrs 20 mins
Accession Number P05067
Regulatory Status RUO
Target Amyloid beta 42
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Detection Antibody,Anti-IgG HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 351
Sample Type Cerebrospinal Fluid,Supernatant,Tissue Homogenates
Label or Dye HRP
8

Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

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9

Invitrogen™ Human IL-6 ELISA Development Kit (TMB), PeproTech®

ELISA

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10

Invitrogen™ Human Calprotectin L1/S100-A8/A9 Complex ELISA Kit

ELISA

EDGE
Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol S100A8, S100A9
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 35 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 35 to 8,000 pg/mL
Test Time 1 hrs 20 mins
Accession Number 60B8Ag; AI323541; AW546964; B8Ag; BEE11; BEE22; Caga; Cagb; calgranulin A; calgranulin B; calgranulin-A; Calgranulin-B; calprotectin; calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; CGLA; CGLB; Chemotactic cytokine CP-10; chemotactic S100 protein; CP-10; Cystic fibrosis antigen; EGK
Regulatory Status RUO
Target Calprotectin
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 6279, 6280
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
11
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Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

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12

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

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Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
13

Invitrogen™ Human MIG/CXCL9 ELISA Kit

ELISA

EDGE
Protein Family GCPR
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 20 pg/mL
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CXCL9 (MIG)
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 20 pg/mL to 6000 pg/mL
Interassay CV <12%
Gene Symbol CMK,Humig,MIG,SCYB9,crg-10
Storage Requirements 2°C to 8°C
Assay Range 8.23 to 6000 pg/mL
Accession Number Q07325
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 4283
Sample Type Plasma,Serum,Supernatant
Synonym CMK, Humig, MIG, SCYB9, crg-10
14

Invitrogen™ Human IFN gamma ELISA Kit

ELISA

EDGE
Measurement Absorbance at 450 nm minus absorbance (at 550 nm)
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Target Species Human
Conjugate HRP
Description Human IFN gamma ELISA Kit
Interassay CV <12%
Intraassay CV <10
Gene Symbol IFG,IFI
Total Assay Time 3 hrs
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity <2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 25.6 to 1000 pg/mL
Test Time 1 hrs 20 mins
Accession Number P01579
Regulatory Status RUO
Target IFN gamma
Specificity No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3458
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
15

Invitrogen™ Human IL1RA ELISA Kit

ELISA

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