PCR Equipment and Supplies
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Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 2X |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Starting Material | DNA |
| For Use With (Application) | Cloning,Hot-start PCR |
Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit
TaqMan™ RNase P Detection Reagents Kit
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
| Polymerase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
| Polymerase | Platinum Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Starting Material | DNA |
| For Use With (Application) | Hot-start PCR |
| Exonuclease Activity | 5' - 3' |
Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.
| No. of Reactions | 100 Reactions |
|---|---|
| Format | Kit |
| Label or Dye | FAM |
| Quantity | 100 reactions |
| Host Cell | E.coli |
| PCR Method | qPCR |
| Product Line | resDNASEQ |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit
Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 10 X |
| Reverse Transcriptase | MultiScribe™ |
| Final Product Type | cDNA |
| Concentration | 10X |
| For Use With (Application) | miRNA analysis |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Size (Final Product) | 200 bp or less |
| Reaction Format | Separate Components |
| Product Type | MicroRNA Reverse Transcription Kit |
| Sample Type | RNA |
| Reaction Speed | Fast |
| Optimal Reaction Temperature | 42°C |
| Product Line | TaqMan |
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
| Format | Kit |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Master Mix |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | High-throughput Compatible |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
| Format | Tube |
| Product Type | Fast Advanced Cells-to-Ct Kit |
| Sample Type | Mammalian cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Purification Target | Lysate |
| Starting Material | 10-100,000 Cells |
| Product Line | Cells-to-CT |
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
| Reaction Format | Separate Components |
|---|---|
| Final Product Type | Double Stranded cDNA |
| Product Type | cDNA Synthesis Kit |
| For Use With (Application) | Cloning |
Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
| Fidelity (vs. Taq) | 1X |
|---|---|
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| Starting Material | DNA |
| Exonuclease Activity | 5' - 3' |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
| Polymerase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Standard PCR |
Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Hot-start PCR |
| Polymerase | Platinum Taq DNA Polymerase |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | Taq DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |