Direct PCR Reagents and Kits
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Filtered Search Results
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
| Format | Kit |
| Sample Type | PCR Products |
| Isolation Technology | Silica Spin Column |
| Run Time | 10 min. |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Shipping Condition | Frozen, Dry Ice |
|---|---|
| Target | cDNA |
| Content And Storage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
| Format | Kit |
| Sample Type | Mammalian Cells |
| Isolation Technology | Direct cell lysis |
| Kit Type | Direct Cell Lysis RT-qPCR |
| Purification Target | RNA |
| Starting Material | 10-100,000 Cells |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | Yes |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | High-throughput Compatible |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
| Format | Tube |
| Product Type | Fast Advanced Cells-to-Ct Kit |
| Sample Type | Mammalian cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Purification Target | Lysate |
| Starting Material | 10-100,000 Cells |
| Product Line | Cells-to-CT |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping,Hot-start PCR |
| PCR Method | Direct PCR |
| Polymerase | Phire Hot Start II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Tissue |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Tube |
| Product Type | 1-Step qPCR kit |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Starting Material | 10-100,000 Cells |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Product Line | Cells-to-CT, TaqMan |
| Detection Method | TaqMan |
Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.
Invitrogen™ RNA Control 250 with Manual
The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.
| Content And Storage | RNA Control and THE RNA Storage Solution should be stored at –20°C |
|---|---|
| Format | Tube |
| Label or Dye | Unconjugated |
| Product Line | Ambion |
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
| Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
|---|---|
| Format | Spin Column |
| Run Time | 5 min. |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit
The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | High-throughput Compatible |
| PCR Method | 2-step RT-qPCR |
| Detection Method | SYBR |
| Format | Tube |
| Product Type | Fast Advanced Cells-to-Ct Kit |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Purification Target | Lysate |
| Starting Material | 10-100,000 Cells |
| Product Line | Cells-to-CT |
Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
| Shipping Condition | Room Temperature |
|---|---|
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
| Content And Storage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
| Format | Kit |
| Product Type | PCR Clean-Up Kit |
| Sample Type | PCR Products |
| Isolation Technology | ChargeSwitch™ Chemistry |
| Run Time | 5 min |
| For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
| High-throughput Compatibility | High-throughput Compatible |
| Product Line | ChargeSwitch |
Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.
Thermo Scientific™ Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | Direct PCR |
| Polymerase | Phusion Blood II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.
| Shipping Condition | Room Temperature |
|---|---|
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
| Content And Storage | The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer. |
| Format | Kit |
| Product Type | PCR Clean-Up Kit |
| Sample Type | PCR Products |
| Isolation Technology | ChargeSwitch™ Chemistry |
| For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Product Line | ChargeSwitch |