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Descripción
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.
Features
- Direct PCR reduces time-consuming and expensive DNA purification steps
- Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
- Specially engineered Phire DNA polymerase for extremely short PCR protocol times
- Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
Notes
- The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
Especificaciones
Especificaciones
| Concentration | 2X |
| Content And Storage | • 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL • Dilution Buffer, 5 mL • DNARelease Additive, 3 x 100 μL • 25 μM Control Primer Mix, 40 μL • Nuclease-free water, 2 x 1.25 mL • 50 μg O'GeneRuler Express DNA Ladder Store at -20°C. |
| GC-Rich PCR Performance | Medium |
| PCR Method | Direct PCR |
| Polymerase | Phire Hot Start II DNA Polymerase |
| Reaction Speed | Fast |
| Includes Label or Dye | Yes |
| Product Type | Direct PCR Master Mix |
| Quantity | 250 reactions |
| Shipping Condition | Wet or Dry Ice |
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Seguridad y manipulación
- Phire Tissue Direct PCR Master Mix/DNARelease Additive
- Danger
- Respiratory sensitiser Category 1
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P501-Dispose of contents/container to .
- MIXTURE LIST-Contains : Proteinase, tritirachium album serine
For Research Use Only. Not for use in diagnostic procedures.
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