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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
DNA Sequencing (194)
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1
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Applied Biosystems™ Hi-Di™ Formamide

Highly deionized (Hi-Di) formamide

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2
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Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

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Shipping Condition Ambient, Wet Ice, Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 6 fragments
Product Type HiFi Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART
3
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Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

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4
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Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

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Antibiotic Resistance Bacterial Ampicillin (AmpR)
Format Kit
Sample Type PCR Products
Cloning Method Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application) PCR Cloning
Vector pJET1.2/blunt
Product Line CloneJET
5
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Thermo Scientific™ pBR322 Plasmid DNA

Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.

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6
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Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

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Format 12-well Strips,8-well Strip,96- or 384-well Plate
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Product Line BigDye™ Terminator
7

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

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Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
8

Applied Biosystems™ 3730 DNA Analyzer 48-Capillary Array, 36 cm

This is a Capillary Array for the Applied Biosystems™ 3730 DNA Analyzer. Each array contains 48 capillaries, 36cm long. Used for both sequencing and fragment analysis runs.

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9
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Applied Biosystems™ Anode Buffer Container Dx

The Anode Buffer Container Dx contains 1X running buffer to support electrophoresis applications on Applied Biosystems Dx genetic analyzers.

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10

Thermo Scientific™ pENTR™/D-TOPO™ Cloning Kit

Utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System

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11
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Thermo Scientific™ Rapid DNA Ligation Kit

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

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Product Type Rapid DNA Ligation Kit
Enzyme DNA Ligase
12
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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Each kit uses the pCR™ 2.1-TOPOTA vector with convenient restriction sites for subcloning. The cloning kits are available with a variety of competent cells (available separately) or without competent cells, depending upon your needs and budget.

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Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Product Line TOPO
13

Applied Biosystems™ 3730xl DNA Analyzer 96-Capillary Array, 50 cm

This is a capillary array for the Applied Biosystems™ 3730XL DNA analyzer.

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14
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Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells

An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.

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Content And Storage Dry Ice (-80°C)
Description Bacterial/Yeast Strain: DH10B
For Use With (Application) Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS)
15
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Applied Biosystems™ Sequencing Standard, BigDye™ Terminator v3.1, for 3500/SeqStudio™ Flex

The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.

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