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Modifying Enzyme Buffers

Reaction buffers for use in experiments involving modifying enzymes. Different buffer solutions create optimal reaction conditions for specific modifying enzymes, or facilitate multiple enzymatic reactions.

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1

5X Kinase Buffer A

5X Kinase Buffer A

Physical Form Liquid
Chemical Name or Material Kinase Buffer A
Concentration 5X
For Use With (Application) Pharma & Biopharma; Protein Assays and Analysis
2

Thermo Scientific™ Kinase buffer I (5X)

For cellular and molecular biology applications

Formulation 100mM Tris-HCl 100, 50mM magnesium chloride, 5mM dithiothreitol, and 5mM sodium orthovanadate, pH 7.4.
Physical Form Liquid
Chemical Name or Material Kinase buffer I (5X)
Recommended Storage Ambient temperatures
3

Thermo Scientific™ Kinase buffer II (5X)

For cellular and molecular biology applications

Formulation 100mM MOPS, 50mM magnesium acetate, 5mM DTT 5, 5mM EDTA, 25mM beta glycerophosphate, and 5mM sodium orthovanadate, pH 7.4
Physical Form Liquid
Chemical Name or Material Kinase buffer II (5X)
Recommended Storage Ambient temperatures
4

Thermo Scientific™ Alkaline Phosphatase Buffer-1 (5X)

For cellular and molecular biology applications

Formulation 200mM Tris, 200mM NaCl, and 40mM magnesium chloride at pH 9.5
Physical Form Liquid
Chemical Name or Material Alkaline Phosphatase Buffer-1 (5X)
Recommended Storage Keep cold
6

Thermo Scientific™ Kinase Buffer T (5X)

Kinase buffer that has been specially optimized for use with the Type III PI4 kinases including PI4KA and PI4KB.

7

Thermo Scientific™ Kinase Buffer R (5X)

Kinase buffer that has been specially optimized for use with the Class I & II PI3 kinases.

9
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Thermo Scientific™ Buffers for RNA Transctription and Post-Transcriptional Modifications

Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.

Physical Form Liquid
Concentration 10X
10

Kinase buffer V (5X), Thermo Scientific™

For cellular and molecular biology applications

Formulation 100mM MOPS, 50mM magnesium chloride, and 50mM manganese chloride, pH 7.4
Physical Form Liquid
Chemical Name or Material Kinase buffer V (5X)
Recommended Storage Ambient temperatures
11

Phosphatase buffer 1 (5X), Thermo Scientific™

For cellular and molecular biology applications

Formulation 100mM Tris (pH 7.5) and 100mM magnesium chloride
Physical Form Liquid
Chemical Name or Material Phosphatase buffer 1 (5X)
Recommended Storage Ambient temperatures
12

Enzyme storage buffer in TBS and glycerol, autoclaved, Thermo Scientific™

For frozen storage of enzymes

Physical Form Liquid
Chemical Name or Material Enzyme storage buffer in TBS and glycerol
Recommended Storage Ambient temperatures