Cell Culture Media, Supplements, and Reagents
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Gibco™ RPMI 1640 Medium, HEPES, no glutamine
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Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
| Form | Liquid |
|---|---|
| Without Additives | No Glutamine,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 12 Months |
| Concentration | 1 X |
Gibco™ Penicillin-Streptomycin (10,000 U/mL)
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For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria
| Sterilization Method | Sterile-filtered |
|---|---|
| Product Type | Antibiotic |
| Form | Liquid |
| Shelf Life | 12 Months |
| Concentration | 100 X |
| For Use With (Application) | Prevention of Cell Culture Contamination |
| Sterility | Sterile-filtered |
Gibco™ Neurobasal™ Medium
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Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement.
Gibco™ TrypLE™ Express Enzyme (1X), phenol red
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Animal origin-free, recombinant enzyme
| Shipping Condition | Room Temperature |
|---|---|
| Chelators | EDTA |
| Form | Liquid |
| Tested For | In vitro Bioassay |
| Concentration | 1 X |
| Osmolality | Check and Record |
| Sterility | Sterile-filtered |
| Source | Animal origin-free |
| Cell Type | Mammalian Cells |
| Remarks | Room Temperature Stable |
| With Additives | Phenol Red,EDTA |
| Product Type | Cell Culture Dissociation Reagent |
| Classification | Animal Origin-free |
| Culture Type | Adherent Cell Culture |
| Shelf Life | 24 Months |
| Product Line | Gibco |
Gibco™ FreeStyle™ F17 Expression Medium
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.
| Cell Line | HEK293 |
|---|---|
| Culture Environment | CO2 |
| Purity or Quality Grade | Qualified for Further Manufacturing |
| Form | Liquid |
| Without Additives | No Glutamine |
| Endotoxin Level | < 1 EU/mL |
| Concentration | 1 X |
| For Use With (Application) | Protein expression, viral vector production |
| Sterility | Sterile |
| Product Type | Expression Medium |
| Classification | Animal Origin-free,Chemically-defined,Protein-free,Serum-free |
| Culture Type | Suspension Cell Culture |
| Product Line | Freestyle |
| Serum Level | Serum-free |
Gibco™ HBSS, with calcium and magnesium; no phenol red
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HBSS, with calcium and magnesium. No phenol red.
| Regulatory Status | In-vitro Diagnostic Use |
|---|---|
| With Additives | Calcium,Magnesium,Glucose,Sodium Bicarbonate |
| Product Type | Hanks' Balanced Salt Solution |
| Form | Liquid |
| Without Additives | No Phenol Red |
| Shelf Life | 36 Months |
| Certifications/Compliance | ISO 13485 standards, FDA |
| For Use With (Application) | Mammalian Cell Culture |
Gibco™ MEM, GlutaMAX™ Supplement
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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
| Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Cell Type | Primary Rat Astrocytes |
| Sterilization Method | Sterile-filtered |
| With Additives | Low Glucose,GlutaMAX,Phenol Red |
| Product Type | MEM (Minimum Essential Medium) |
| Classification | Animal Origin-free |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | Gibco, GlutaMAX |
| Serum Level | Standard Serum Supplementation |
Gibco™ Ham's F-12 Nutrient Mix
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Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.
| Cell Line | CHO, COS-7, and rat prostate epithelial cells |
|---|---|
| Sterilization Method | Sterile-filtered |
| With Additives | Glutamine,Phenol Red,Sodium Pyruvate |
| Product Type | Ham's F-12 Nutrient Mixture |
| Form | Liquid |
| Without Additives | No HEPES |
| Classification | Animal Origin-free |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 12 Months From Date of Manufacture |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Product Line | Gibco |
| Cell Type | Primary Rat Astrocytes |
Gibco™ Advanced DMEM
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Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero |
| Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Form | Liquid |
| Without Additives | No Glutamine,No HEPES |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA) |
| Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
| Classification | Animal Origin |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | Gibco |
| Serum Level | Reduced Serum |
Keratinocyte-SFM Medium (Kit) with L-glutamine, EGF, and BPE
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Gibco™ MEM, no glutamine
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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MEM, no glutamine
| Regulatory Status | In-vitro Diagnostic Use |
|---|---|
| Content And Storage | 2 to 8°C |
| Description | MEM Medium, 500mL, 1 X Concentration, Liquid Form, With Phenol Red, 7 to 7.4 pH, 12 Months Shelf Life, 280 to 320mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Soluble in Water, Used for Mammalian Cell Culture |
| With Additives | Phenol Red |
| Product Type | MEM Medium |
| Form | Liquid |
| Without Additives | No Glutamine,No HEPES |
| pH | 7 to 7.4 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ Sf-900™ II SFM
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Optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells
| Cell Line | Sf21, Sf9 |
|---|---|
| Content And Storage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| With Additives | Glutamine |
| Product Type | Insect Cell Serum Free Medium (SFM) |
| Form | Liquid |
| Classification | Protein-free,Serum-free |
| Species | S. frugiperda, Spodoptera frugiperda |
| Product Line | Gibco, Sf-900 |
| Serum Level | Serum-free |
| Cell Type | Insect Cell |
Gibco™ CTS™ Essential 8™ Medium
CTS Essential 8 Medium is a fully-defined, feeder-free medium designed for the growth and expansion of hPSCs for clinical applications and formulated with components not directly derived from animals.
Gibco™ Medium 199, GlutaMAX™ Supplement
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Medium 199, GlutaMAX™ Supplement
| Content And Storage | 2 to 8°C |
|---|---|
| With Additives | GlutaMAX,Phenol Red,Sodium Bicarbonate |
| Product Type | Medium 199 |
| Form | Liquid |
| Without Additives | No HEPES |
| pH | 7.1 to 7.4 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Cultivation of non-transformed cells, mammalian cell culture |
Gibco™ Medium 254
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Medium 254