Cell Culture Media, Supplements, and Reagents
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Gibco™ RPMI 1640 Medium, HEPES, no glutamine
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
| Form | Liquid |
|---|---|
| Without Additives | No Glutamine,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 12 Months |
| Concentration | 1 X |
Gibco™ Penicillin-Streptomycin (10,000 U/mL)
For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria
| Sterilization Method | Sterile-filtered |
|---|---|
| Product Type | Antibiotic |
| Form | Liquid |
| Shelf Life | 12 Months |
| Concentration | 100 X |
| For Use With (Application) | Prevention of Cell Culture Contamination |
| Sterility | Sterile-filtered |
| Content And Storage | 2 to 8°C |
|---|---|
| Product Type | Sodium Pyruvate |
| Form | Liquid |
| Shelf Life | 12 Months |
| Certifications/Compliance | FDA, ISO 13485 |
| For Use With (Application) | Mammalian Cell Culture |
Gibco™ TrypLE™ Express Enzyme (1X), phenol red
Animal origin-free, recombinant enzyme
| Shipping Condition | Room Temperature |
|---|---|
| Chelators | EDTA |
| Form | Liquid |
| Tested For | In vitro Bioassay |
| Concentration | 1 X |
| Osmolality | Check and Record |
| Sterility | Sterile-filtered |
| Source | Animal origin-free |
| Cell Type | Mammalian Cells |
| Remarks | Room Temperature Stable |
| With Additives | Phenol Red,EDTA |
| Product Type | Cell Culture Dissociation Reagent |
| Classification | Animal Origin-free |
| Culture Type | Adherent Cell Culture |
| Shelf Life | 24 Months |
| Product Line | Gibco |
Gibco™ DMEM, no glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ DMEM, low glucose, GlutaMAX™ Supplement, pyruvate
DMEM, low glucose, GlutaMAX™ Supplement, pyruvate
| Regulatory Status | In-vitro Diagnostic Use |
|---|---|
| Content And Storage | Store at 2–8°C. |
| Description | DMEM Medium, 500mL, 1 X Concentration, Liquid Form, With Low Glucose, Sodium Pyruvate, GlutaMAX™, Phenol Red, 7 to 7.2 pH, 12 Months Shelf Life, 300 to 340mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture |
| With Additives | Low Glucose,Sodium Pyruvate,GlutaMAX,Phenol Red |
| Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
| Form | Liquid |
| Without Additives | No HEPES |
| pH | 7 to 7.2 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ IMDM, GlutaMAX™ Supplement
IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.
| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | Jurkat, COS-7, and macrophage cells |
| Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Form | Liquid |
| Without Additives | No α-Thioglycerol,No 2-Mercaptoethanol |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | High Glucose,GlutaMAX,HEPES,Phenol Red,Sodium Pyruvate |
| Product Type | IMDM (Iscove's Modified Dulbecco's Medium) |
| Classification | Animal Origin-free |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | Gibco, GlutaMAX |
Gibco™ FreeStyle™ F17 Expression Medium
An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.
| Cell Line | HEK293 |
|---|---|
| Culture Environment | CO2 |
| Purity or Quality Grade | Qualified for Further Manufacturing |
| Form | Liquid |
| Without Additives | No Glutamine |
| Endotoxin Level | < 1 EU/mL |
| Concentration | 1 X |
| For Use With (Application) | Protein expression, viral vector production |
| Sterility | Sterile |
| Product Type | Expression Medium |
| Classification | Animal Origin-free,Chemically-defined,Protein-free,Serum-free |
| Culture Type | Suspension Cell Culture |
| Product Line | Freestyle |
| Serum Level | Serum-free |
| Content And Storage | 2 to 8°C |
|---|---|
| With Additives | Glutamine,Phenol Red |
| Product Type | MEM Medium |
| Form | Liquid |
| Without Additives | No HEPES |
| pH | 7 to 7.4 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ HBSS, with calcium and magnesium; no phenol red
HBSS, with calcium and magnesium. No phenol red.
| Regulatory Status | In-vitro Diagnostic Use |
|---|---|
| With Additives | Calcium,Magnesium,Glucose,Sodium Bicarbonate |
| Product Type | Hanks' Balanced Salt Solution |
| Form | Liquid |
| Without Additives | No Phenol Red |
| Shelf Life | 36 Months |
| Certifications/Compliance | ISO 13485 standards, FDA |
| For Use With (Application) | Mammalian Cell Culture |
Gibco™ McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.
| Form | Liquid |
|---|---|
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Regulatory Status | In-vitro Diagnostic Use |
|---|---|
| Product Type | RPMI 1640 Medium |
| Form | Liquid |
| Certifications/Compliance | ISO 13485 standards, FDA |
| For Use With (Application) | Mammalian cell culture |
| Content And Storage | 2 to 8°C |
|---|---|
| With Additives | GlutaMAX,Phenol Red |
| Product Type | Minimum Essential Medium |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| pH | 7.0 to 7.4 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ MEM α, no nucleosides
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
| Sterilization Method | Sterile-filtered |
|---|---|
| Product Type | MEM α (Minimum Essential Medium α) |
| Form | Liquid |
| Classification | Animal Origin-free |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1X |
| Sterility | Sterile-filtered |
| Product Line | Gibco |
| Serum Level | Standard Serum Supplementation |
Gibco™ Cell Dissociation Buffer, enzyme-free, PBS
Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).