Cell Culture Media, Supplements, and Reagents
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Gibco™ RPMI 1640 Medium, HEPES, no glutamine
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
| Form | Liquid |
|---|---|
| Without Additives | No Glutamine,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 12 Months |
| Concentration | 1 X |
Gibco™ B-27™ Supplement (50X), serum free
The original Gibco B-27 Supplement, optimized for serum-free culture of neurons, supports the growth and viability of neurons and many different cell types.
| Content And Storage | Store in freezer (-5 to -20°C) and protect from light. |
|---|---|
| Product Type | Cell Culture Supplement |
| Form | Liquid |
| Tested For | Endotoxin, Performance, Sterility, Mycoplasma |
| Classification | Serum-free |
| Culture Type | Mammalian Cell Culture |
| Shelf Life | 12 Months |
| Sterility | Sterile |
| Serum Level | Serum-Free |
| Cell Type | Neural Cells |
Gibco™ Trypsin-EDTA (0.25%), phenol red
Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas
| Chelators | EDTA |
|---|---|
| Content And Storage | Storage conditions: -5°C to -20°C Shipping conditions: Wet ice Shelf life: 24 months from date of manufacture |
| Form | Liquid |
| Tested For | In vitro Bioassay |
| Osmolality | 270 - 320 mOsm/kg |
| Sterility | Sterile-filtered |
| Source | Animal Origin |
| Cell Type | Mammalian Cells |
| Sterilization Method | Sterile-filtered |
| With Additives | Phenol Red,EDTA |
| Product Type | Cell Culture Dissociation Reagent |
| Classification | Animal Origin |
| Shelf Life | 24 Months |
| Product Line | Gibco |
Gibco™ Trypsin-EDTA (0.05%), phenol red
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.
| Chelators | EDTA |
|---|---|
| Sterilization Method | Sterile-filtered |
| With Additives | Phenol Red,EDTA |
| Product Type | Cell Culture Dissociation Reagent |
| Form | Liquid |
| Tested For | In vitro Bioassay |
| Classification | Animal Origin |
| Shelf Life | 24 Months |
| Osmolality | 270 - 310 mOsm/kg |
| Sterility | Sterile-filtered |
| Source | Animal Origin |
| Product Line | Gibco |
| Cell Type | Mammalian Cells |
Gibco™ N-2 Supplement (100X)
N-2 Supplement is a versatile serum-free cell culture supplement that supports the growth and viability of various cell types.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | Store in freezer (-5 to -20°C) and protect from light. |
| Product Type | Cell Culture Supplement |
| Form | Liquid |
| Tested For | Endotoxin, Performance, Sterility |
| Classification | Serum-free |
| pH | 6 to 8 |
| Culture Type | Mammalian Cell Culture |
| Shelf Life | 18 Months |
| Concentration | 100 X |
| Sterility | Sterile |
| Serum Level | Serum-Free |
| Cell Type | Neural Cells |
Gibco™ RPMI 1640 Medium (ATCC modification)
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
| Shipping Condition | Ambient |
|---|---|
| Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
| Content And Storage | Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 18 months from date of manufacture |
| Form | Liquid |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1X |
| Sterility | Sterile-filtered |
| Cell Type | Mammalian Cells |
| Sterilization Method | Sterile-filtered |
| With Additives | Glutamine,Phenol Red |
| Product Type | Opti-MEM I |
| Classification | Human Origin |
| Shelf Life | 18 Months From Date of Manufacture |
| Product Line | Gibco, Opti-MEM |
Gibco™ GlutaMAX™ Supplement
GlutaMAX Supplement is an L-alanyl-L-glutamine dipeptide alternative to L-glutamine. It exhibits increased stability over L-glutamine leading to improved cellular health.
| Content And Storage | Storage conditions: 15°C to 30°C Alternate storage conditions: -5°C to -20°C and 2°C to 8°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
|---|---|
| Product Type | GlutaMax |
| Form | Liquid |
| Classification | Animal Origin-free |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Culture Type | Mammalian Cell Culture |
| Shelf Life | 24 Months |
| For Use With (Application) | Clinical Research, Translational Research |
| Sterility | Sterile-filtered |
| Product Line | Gibco, GlutaMAX |
| Cell Type | Mammalian Cells |
Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement
RPMI 1640 Medium, GlutaMAX™ Supplement
| Content And Storage | 2 to 8°C |
|---|---|
| With Additives | GlutaMAX,Phenol Red |
| Product Type | Roswell Park Memorial Institute (RPMI) 1640 Medium |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| pH | 7.0 to 7.4 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ MEM Non-Essential Amino Acids Solution (100X)
MEM Non-Essential Amino Acids Solution (100X)
| Content And Storage | 2 to 8°C |
|---|---|
| Product Type | Amino Acid Supplement |
| Shelf Life | 12 Months |
| Concentration | 100 X |
| Certifications/Compliance | FDA, ISO 13485 |
| For Use With (Application) | Mammalian Cell Culture |
Gibco™ TrypLE™ Express Enzyme (1X), no phenol red
Unlike porcine trypsin, free of animal-derived components
| Shipping Condition | Room Temperature |
|---|---|
| Chelators | EDTA |
| Form | Liquid |
| Without Additives | No Phenol Red |
| Tested For | In vitro Bioassay |
| Concentration | 1 X |
| Osmolality | Check and Record |
| Sterility | Sterile-filtered |
| Source | Animal origin-free |
| Cell Type | Mammalian Cells |
| Remarks | Room Temperature Stable |
| Product Type | Cell Culture Dissociation Reagent |
| Classification | Animal Origin-free |
| Culture Type | Adherent Cell Culture |
| Shelf Life | 24 Months |
| Product Line | Gibco |
| Regulatory Status | In-vitro Diagnostic Use |
|---|---|
| Content And Storage | –5 to –20°C |
| Product Type | L-Glutamine |
| Form | Liquid |
| Shelf Life | 18 Months |
| Concentration | 100 X |
| Certifications/Compliance | cGMP, ISO 13485 standard, FDA |
| For Use With (Application) | Mammalian Cell Culture |
Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate
DMEM, high glucose, GlutaMAX™ Supplement, pyruvate
| Content And Storage | 2 to 8°C |
|---|---|
| With Additives | High Glucose,Sodium Pyruvate,GlutaMAX,Phenol Red |
| Product Type | Dulbecco's Modified Eagle Medium |
| Form | Liquid |
| Without Additives | No HEPES |
| pH | 6.8 to 7.2 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Tissue and cell culture processing, mammalian cell culture |
Gibco™ B-27™ Supplement (50X), minus vitamin A
A variant of the original serum-free B-27 Supplement, designed for the proliferation of neural stem cells in the absence of vitamin A.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | Store in freezer (-5 to -20°C) and protect from light. |
| Product Type | Cell Culture Supplement |
| Form | Liquid |
| Classification | Serum-free |
| Culture Type | Mammalian Cell Culture |
| Shelf Life | 12 Months |
| Concentration | 50 X |
| Sterility | Sterile |
| Source | Serum-Free |
| Product Line | B-27 |
| Serum Level | Serum-Free |
| Cell Type | Neural Progenitor Cells |
Gibco™ DMEM/F-12
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts |
| Content And Storage | Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Form | Liquid |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
| Sterilization Method | Sterile-filtered |
| With Additives | High Glucose,Glutamine,Phenol Red,Sodium Pyruvate |
| Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
| Classification | Animal Origin-free |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | Gibco |
| Serum Level | Standard Serum Supplementation |