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Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
| Product Type | Alkaline Phosphatase |
|---|---|
| Enzyme | Phosphatase |
| Compatible Buffer | PCR Buffer, Enzyme Buffer |
| Concentration | 1 U/μL |
Thermo Scientific™ FastDigest BglII
Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bgl II |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | FastDigest™ |
Thermo Scientific™ FastDigest LguI (IIs class)
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
| Content And Storage | Uracil-DNA glycolase (1 U/μL) and 10X Reaction Buffer. Store at -20°C. |
|---|---|
| Product Type | Uracil DNA Glycosylase |
| Enzyme | Uracil-DNA Glycosylase |
| Compatible Buffer | 10X Reaction Buffer |
| Concentration | 1 U/μL |
Thermo Scientific™ BglII (10 U/μL)
Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
| Product Type | TdT |
|---|---|
| Enzyme | Terminal Deoxynucleotidyl Transferase |
| Compatible Buffer | Storage Buffer, 5X Reaction Buffer |
| Concentration | 20 U/μL |
Thermo Scientific™ FastDigest XmaJI
Cut at C^CTAGG sites with Thermo Scientific™ FastDigest™ AvrII (XmaJI), which performs best using universal FastDigest Buffer (Isoschizomers: AspA2I, BlnI).
Thermo Scientific™ Kpn2I (BspEI) (10 U/μL)
The Kpn2I (BspEI) restriction enzyme recognizes T^CCGGA sites and cuts best at 55°C in Tango buffer (Isoschizomers: AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Kpn2I (BspEI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 55°C |
| Concentration | 10 U/μL |
Thermo Scientific™ PagI (BspHI) (10 U/μL)
The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PagI (BspHI) |
| Methylation Sensitivity | Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer O |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest Pfl23II
Cut at C^GTACG sites with Thermo Scientific™ FastDigest™ BsiWI (Pfl23II), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspLI).
Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ SgsI (AscI) (10 U/μL)
The SgsI (AscI) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AscI, PalAI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SgsI (AscI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ Alw44I (10 U/μL)
Cut at G^TGCAC sites with Alw44I (ApaLI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ApaLI, VneI).
Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)
Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | Store at -20°C. |
| Host Species | T. album |
| Form | Liquid |
| Product Type | Proteinase K |
| Concentration | 20 mg/mL |
| Product Line | Ambion™ |