PCR Equipment and Supplies
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Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | SYBR |
| Detection Method | SYBR |
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ TaqMan™ RNA-to-CT™ 1-Step Kit
Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Reverse Transcriptase | ArrayScript™ UP |
| Product Type | One-Step RT-PCR Kit |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | RNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Product Line | RNA-to-CT, TaqMan |
| Detection Method | Primer-probe |
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 to 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
| Polymerase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Standard PCR |
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.
| Purity or Quality Grade | UP (Ultra Pure) |
|---|---|
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Bottle |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode |
| Sample Type | Environmental Sample, Food |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Invitrogen™ Oligo(dT)20 Primer
Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C, Oligo(dT)20 Primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA.
Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.
| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 2 X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq DNA Polymerase |
| Format | Tube |
| Product Type | Fast Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Product Line | AmpErase, TaqMan |
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
| Shipping Condition | Wet Ice |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Reverse Transcriptase | ArrayScript™ UP |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Sample Type | RNA |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Run Time | Standard |
| Product Line | RNA-to-CT, SYBR |
Thermo Scientific™ DreamTaq™ Buffers (10X)
Thermo Scientific 10X DreamTaq Buffer is specifically optimized for PCR using DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase.
| Quantity | 4 x 1.25 mL |
|---|---|
| Concentration | 10X |
| For Use With (Application) | Hot-start PCR |
Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity
Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | AccuPrime Taq DNA Polymerase High Fidelity |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 9X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
| Detection Method | Primer-probe |