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Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Life Science Buffers

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

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PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

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Specifications

Life Science Buffers

Physical Form	Liquid
Recommended Storage	Room temperature

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TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Specifications

Life Science Buffers

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

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HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol

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Specifications

Life Science Buffers

Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
Color	White
Chemical Name or Material	HEPES
Grade	Molecular Biology
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol	Gray
Assay Percent Range	≥99 %
PubChem CID	23831
Absorbance	0.01 max. (0.1M solution) at 280nm
Percent Purity	≥99%
CAS	7365-45-9
Protease	Protease free
Health Hazard 3	Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1
MDL Number	MFCD00006158
Health Hazard 2	WARNING!
Solubility Information	Soluble in water
pH	5.0 to 6.5
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C8H18N2O4S

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Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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Life Science Buffers

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Gibco™ HBSS, no calcium, no magnesium

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

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Specifications

Life Science Buffers

Includes	No Sodium Pyruvate
Physical Form	Liquid
pH	6.7 to 7.8
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Hanks' Balanced Salt Solution (HBSS)
For Use With (Application)	Mammalian Cell Culture
Sterility	Sterile-filtered
Solution Type	Hank's Balanced Salt
Product Line	Gibco

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Gibco™ HBSS, calcium, magnesium

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

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Life Science Buffers

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Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

Life Science Buffers

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Tris-HCl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

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Tris Hydrochloride, 1M Solution (pH 7.5/Mol. Biol.), Fisher BioReagents™

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Specifications

Life Science Buffers

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Tris-HCl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

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HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™

Commonly used buffering agent

Pricing and Availability
Specifications

Life Science Buffers

Color	Undesignated
Physical Form	Liquid
Chemical Name or Material	HEPES Buffer
Grade	Molecular Biology
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
Concentration	0.95 to 1.05 M
ChemAlert Storage Symbol	Gray
Absorbance	≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm
Name Note	1M Solution, pH 7.3
CAS	7732-18-5
Protease	Protease free
pH	7.2 to 7.5
Synonym	N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C₈H₁₈N₂O₄S
Formula Weight	238.197

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Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.

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Specifications

Protein Method Blocking Buffers

Physical Form	Liquid
Recommended Storage	Upon receipt store product at room temperature. After opening, store product at 4°C.
Concentration	1X
For Use With (Application)	ELISA

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PBS, Phosphate Buffered Saline, 1X Powder, pH 7.4, Fisher BioReagents

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Specifications

Life Science Buffers

Color	White
Packaging	Poly Bottle
Physical Form	Powder
Recommended Storage	Room temperature

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Invitrogen™ Tris (1 M), pH 8.0, RNase-free

Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.

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Specifications

Life Science Buffers

Formulation	1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride
Physical Form	Liquid
pH	8
Grade	Molecular Biology
Recommended Storage	Store at room temperature.
Concentration	1 M
Product Line	Ambion

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Invitrogen™ Digitonin (5%)

Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.

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Gel Electrophoresis Buffers and Diluents

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Gibco™ PB-MAX™ Karyotyping Medium

Fully supplemented medium developed for short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures

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Specifications

Life Science Buffers

Includes	L-Glutamine
Color	Red
Physical Form	Liquid
Chemical Name or Material	Karyotyping Medium
Recommended Storage	Storage conditions: -5 to -20°C. Protect from light. Shipping conditions: Frozen Shelf life: 12 months from date of manufacture
Concentration	1 X
For Use With (Application)	Cell Viability; Proliferation & Function; Clinical & Translational Research; Mammalian Cell Culture
Sterility	Sterile-filtered
Product Line	PB-MAX

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Gibco™ HBSS, no calcium, no magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ HBSS, calcium, magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PB-MAX™ Karyotyping Medium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ HBSS, no calcium, no magnesium

Gibco™ HBSS, calcium, magnesium

Gibco™ PB-MAX™ Karyotyping Medium