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Buffers and Standards

Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.

The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:

  • Red: pH 4.0
  • Yellow: pH 7.0
  • Blue: pH 10.0

 

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1 – 15 of 21,508 results
1

Buffer Solution, pH 10.00, Reagecon™
BIOPHARMA

Certified by an ISO 17025 accredited test method and fully NIST traceable. Reagecon™ Buffer Solution, pH 10.00 is tested at 20°C or 25°C to an accuracy level of ±0.01. This calibration and control buffer is specially formulated to ensure its stability, even after opening the bottle.

EDGE
Boiling Point 100°C
Color Colorless
Solubility Information Fully miscible in water
Traceability to NIST Yes
Physical Form Liquid
Odor Characteristic
Solution Type pH Buffer
Melting Point 0°C
2
Promotions Available

Gibco™ DPBS, no calcium, no magnesium
GREENER_CHOICE

DPBS, no calcium, no magnesium

EDGE
Physical Form Liquid
Chemical Name or Material DPBS
Recommended Storage 15°to 30°C
Concentration 1 X
3
Promotions Available

Thermo Scientific™ Orion™ pH Buffer Bottles

Perform accurate, reproducible pH calibrations with these color-coded buffer bottles.

EDGE
For Use With (Equipment) pH Meter and Electrode
Traceability to NIST Yes
Physical Form Liquid
Chemical Name or Material pH Buffer, bottles
4
Promotions Available

pH Buffers, Twin Neck, Fisherbrand™

Perform accurate pH calibrations with color-coded, twin neck Fisherbrand™ pH Buffer Bottles.

EDGE
For Use With (Application) pH calibration
5
Promotions Available

Buffer Solution pH7.00 (Phosphate), NIST Standard Solution ready to use for pH measurement, Fisher Chemical™

MDL Number: 217672

EDGE
MDL Number 217672
6
Promotions Available

Buffer Solution pH4.00 (Phthalate), NIST Standard Solution ready to use for pH measurement, Fisher Chemical™

MDL Number: 217672

EDGE
MDL Number 217672
7
Promotions Available

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

EDGE
Physical Form Liquid
Recommended Storage Room temperature
8

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

EDGE
Color Colorless
Physical Form Liquid
Chemical Name or Material Tris-EDTA
Grade Molecular Biology
DNase DNase free
Filtered Through Filtered through a 5-micron filter.
ChemAlert Storage Symbol Gray
Name Note 1X Solution, pH 8.0
CAS 7732-18-5
Protease Protease free
Health Hazard 3 Emergency Overview
May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention.

NFPA

Health:1
Flammability:0
Instability:0
Health Hazard 2 CAUTION!
pH 7.4 to 8.1
Synonym TE
Recommended Storage RT
9
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TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

EDGE
Boiling Point 100°C
Color Colorless
Physical Form Liquid
Chemical Name or Material Tris Buffered Saline
Grade Molecular Biology
DNase DNase free
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS 7732-18-5
Protease Protease free
pH 7.5
Synonym TBS
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
10
Promotions Available

Thermo Scientific™ Orion™ pH Buffer Individual Use Pouches

Ensure fresh, ready-to-use standards for pH calibration with these pH Buffer individual use pouches.

EDGE
Boiling Point 100°C
For Use With (Equipment) pH Meter and Electrode, single-use
Solubility Information Soluble
Traceability to NIST Yes
Packaging Pouch
Physical Form Liquid
Chemical Name or Material pH Buffer, individual use pouches
For Use With (Application) pH calibration
Solution Type Buffer
11
Promotions Available

Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

EDGE
PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
SMILES C(C(CO)(CO)N)O
Synonym Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
12
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HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol

EDGE
Molecular Weight (g/mol) 238.30
ChEBI CHEBI:42334
Color White
Chemical Name or Material HEPES
Grade Molecular Biology
SMILES OCCN1CCN(CCS(O)(=O)=O)CC1
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol Gray
Assay Percent Range ≥99 %
PubChem CID 23831
Absorbance 0.01 max. (0.1M solution) at 280nm
Percent Purity ≥99%
CAS 7365-45-9
Protease Protease free
Health Hazard 3 Emergency Overview
Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. .

NFPA

Health:2
Flammability:1
Instability:1
MDL Number MFCD00006158
Health Hazard 2 WARNING!
Solubility Information Soluble in water
pH 5.0 to 6.5
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
13
Promotions Available

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

EDGE
14
Promotions Available

Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

EDGE
PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
SMILES C(C(CO)(CO)N)O
Synonym Tris-HCl
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
15

Ethylenediaminetetraacetic Acid (0.5M Solution/pH 8.0), Fisher BioReagents

CAS: 60-00-4 Molecular Formula: C10H16N2O8 Molecular Weight (g/mol): 292.24 MDL Number: MFCD00003541 InChI Key: KCXVZYZYPLLWCC-UHFFFAOYSA-N Synonym: edta,edetic acid,ethylenediaminetetraacetic acid,edathamil,versene,endrate,havidote,titriplex,edta acid,sequestrol PubChem CID: 6049 ChEBI: CHEBI:42191 IUPAC Name: 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid SMILES: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

EDGE
PubChem CID 6049
CAS 60-00-4
Molecular Weight (g/mol) 292.24
ChEBI CHEBI:42191
MDL Number MFCD00003541
SMILES OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
Synonym edta,edetic acid,ethylenediaminetetraacetic acid,edathamil,versene,endrate,havidote,titriplex,edta acid,sequestrol
IUPAC Name 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid
InChI Key KCXVZYZYPLLWCC-UHFFFAOYSA-N
Molecular Formula C10H16N2O8