Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Thermo Scientific™ ELISA Carbonate Coating Buffer
For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.
Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate
HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.
| Content And Storage | Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction |
|---|---|
| Substrate Properties | Chemical Substrate |
| Product Type | ELISA Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Product Line | SuperSignal |
| Detection Method | Chemiluminescence |
Invitrogen™ Cell Lysis Buffer II
Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.
Thermo Scientific™ SuperSignal™ ELISA Femto Substrate
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
| Content And Storage | Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature. |
|---|---|
| Substrate Properties | Chemical Substrate |
| Product Type | ELISA Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Product Line | SuperSignal |
| Detection Method | Chemiluminescence |
Invitrogen™ Tissue Extraction Reagent I
Lysis buffer for total protein extraction from tissue samples
| Measurement | Absorbance at 450 nm minus absorbance (at 550 nm) |
|---|---|
| Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
| Target Species | Human |
| Conjugate | HRP |
| Description | Human IFN gamma ELISA Kit |
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IFG,IFI |
| Total Assay Time | 3 hrs |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <2 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 25.6 to 1000 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | P01579 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Specificity | No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Precision | Intra-assay CV ≤ 10%; inter-assay CV ≤ 10% |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3458 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 8.1% |
| Intraassay CV | 4.7 |
| Incubation Time | 4 hr. |
| Gene Symbol | MVCD1,VEGF,VPF |
| Total Assay Time | 4 hrs |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <5 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 23.4 to 1500 pg/mL |
| Marker Gene | VEGF |
| For Use With (Application) | Colorimetric Detection |
| Test Time | 1 hrs 20 mins |
| Accession Number | P15692 |
| Regulatory Status | RUO |
| Target | VEGF |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 7422 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Mmp12 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.04 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.04 to 10 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 3.4.24.65; AV378681; HME; Macrophage elastase; Macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); matrix metalloproteinase-12; ME; MGC138506; Mme; Mmel; MMP; MMP12; MMP-12; MMPs |
| Regulatory Status | RUO |
| Target | MMP12 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4321 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.5% |
| Intraassay CV | 4.6 |
| Incubation Time | 4 hr. |
| Gene Symbol | DDPAC,FTDP-17 L,MSTD,MTBT1,MTBT2,PPND,PPP1R103,TAU,MAPT |
| Total Assay Time | 4 hrs |
| Sample Volume | Cerebrospinal Fluid 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <10 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Concentration | 31 to 2000 pg/mL |
| Assay Range | 31 to 2000 pg/mL |
| Marker Gene | Tau |
| Test Time | 1 hrs 20 mins |
| Accession Number | P10636 |
| Regulatory Status | RUO |
| Target | Tau |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4137 |
| Sample Type | Cerebrospinal Fluid,Supernatant |
| Label or Dye | HRP |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 5.6% |
| Intraassay CV | 3.3 |
| Incubation Time | 4 hr. |
| Gene Symbol | CLG4,CLG4A,MMP-2,MMP-II,MONA,TBE-1 |
| Total Assay Time | 4 hrs |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <1 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.78 to 50 ng/mL |
| Marker Gene | MMP-2 |
| For Use With (Application) | Colorimetric Detection |
| Test Time | 1 hrs 20 mins |
| Accession Number | P08253 |
| Regulatory Status | RUO |
| Target | MMP2 |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4313 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |