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  7. Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Product Code. 10659009 Shop All Thermo Scientific Products
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Product Code. No. of Reactions Unit Size
10659009 500 Reactions 500 reactions
10043967 100 Reactions 100 reactions
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Product Code. 10659009 Supplier Thermo Scientific™ Supplier No. F548L
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Description

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols. The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less). The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features

  • Extreme speed with extension times of 15 s/kb or less
  • Hot start modification allows 'zero-time reactivation'
  • Accurate proofreading DNA polymerase with a fidelity of 25X Taq
  • High yields in reduced cycling time
  • Minimized pipetting steps

Applications

  • Fast PCR
  • High-fidelity PCR
  • Difficult (GC-rich) templates
  • Template generation for sequencing Multiplex PCR
  • Long-range PCR
  • Cloning & mutagenesis
  • Microarray

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
TRUSTED_SUSTAINABILITY

Spécification

Concentration 2X
Content And Storage Phusion Flash High-Fidelity PCR Master Mix (5 x 1 mL)

Store at -20°C.
GC-Rich PCR Performance High
Polymerase Phusion Flash II DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity PCR Master Mix
Quantity 500 reactions
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq) 25X
Hot Start Built-In Hot Start
No. of Reactions 500 Reactions
Overhang Blunt
Reaction Format SuperMix or Master Mix
Size (Final Product) 20 kb or less
Color Colorless
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For Research Use Only. Not for use in diagnostic procedures.

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