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Thermo Scientific™ 1-Step™ Chloronaphthol Substrate Solution

Product Code. 11809260 Shop All Thermo Scientific Products
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250 mL
Unit Size:
250mL
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11809260 250 mL 250mL
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Product Code. 11809260 Supplier Thermo Scientific™ Supplier No. 34012

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Description

1-Step™ Chloronaphthol Substrate Solution

Thermo Scientific Pierce 1-Step Chloronaphthol Solution is a solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples. The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB. However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications.

Features of Chloronaphthol Substrates:

  • HRP substrate—for detection of horseradish peroxidase activity on solid media
  • Chromogenic—no special equipment needed for visualization
  • Blue-purple—easy to photograph in color or black-and-white
  • Package options—choose powder or ready-to-use solution

    Highlights of Pierce CN Solution
  • Ready-to-use—no ethanol or methanol required to dissolve; no dilution necessary for use
  • Complete—does not require buffer or addition of hydrogen peroxide
  • Pre-filtered—does not need to be filtered before use like powders or tablets after they are dissolved
  • Stable—store refrigerated at 4°C for at least one year

    • TRUSTED_SUSTAINABILITY

    Specifications

    Product Type Substrate
    Detection Method Colorimetric
    Form Liquid
    Product Line 1-Step
    Quantity 250 mL
    Substrate Horseradish Peroxidase
    Reactivity Horseradish Peroxidase
    Content And Storage Store at 4°C.

    Safety and Handling

    compliance-icons compliance-icons
    Product Identifier
    • 1-Step Chloronaphthol Substrate Solution
     Signal Word
    • Warning
     Hazard Category
    • Acute toxicity Category 4
    • Specific target organ toxicity Category 2
     Hazard Statement
    • H302-Harmful if swallowed.
    • H371-May cause damage to organs.
     Precautionary Statement
    • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
    • P264-Wash hands thoroughly after handling.
    • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
    • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
    • P405-Store locked up.
    • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
     Supplemental information
    • MIXTURE LIST-Contains : methanol

    For Research Use Only. Not for use in diagnostic procedures.

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