missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. Western Blot Substrates and Substrate Kits
  5. 10066433

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Product Code. 10066433 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
250 mL
Unit Size:
250mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10066433 250 mL 250mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10066433 Supplier Thermo Scientific™ Supplier No. 34018

Please to purchase this item. Need a web account? Register with us today!

183.60 EUR valid until 2026-03-27
BEST PRICE on Promo! Use promo code "27964" to get your promotional price.
This item is not returnable. View return policy

Description

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.

TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.

Highlights:

  • HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media

  • Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates

  • Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use

  • Complete – does not require other buffers or addition of hydrogen peroxide

TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Detection Method Colorimetric
Form Liquid
Product Line 1-Step
Quantity 250 mL
Substrate Horseradish Peroxidase
Reactivity Horseradish Peroxidase
Content And Storage Upon receipt store product at 4°C. Product is shipped at ambient temperature.

Safety and Handling

compliance-icons compliance-icons
Product Identifier
  • 1-Step TMB-Blotting, 250 mL
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • Specific target organ toxicity Category 2
 Hazard Statement
  • H302-Harmful if swallowed.
  • H371-May cause damage to organs.
 Precautionary Statement
  • P270-Do not eat, drink or smoke when using this product.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
  • P308+P311-IF exposed or concerned: Call a POISON CENTER/doctor/.
 Supplemental information
  • MIXTURE LIST-Contain: Methyl alcohol

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.