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Description
Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.
Usage Recommendations
- Use fresh 1X TAE both for the gel and for the electrophoresis run
- To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well
Quality Control
- The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
1X Composition
- 40mM Tris
- 20mM acetic acid
- 1mM EDTA
- pHof 50X TAE: 8.4
Recommended for:
Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.
Note:
TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.
Specifications
Specifications
| Components | 40mM Tris; 20mM acetic acid; 1mM EDTA; pHof 50X TAE: 8.4 |
| Content And Storage | There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating. |
| Quantity | 1 L |
| Product Type | 50X TAE Buffer (Tris-acetate-EDTA) |
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