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Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
No need for an RNA purification step
Brand: Invitrogen™ 11753100
Additional Details : Weight : 0.05000kg
Description
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
- Efficiency: offers significant time and cost savings for increased throughput
- Convenience: enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
- Sensitivity: no separate RNA isolation step increases sensitivity by minimizing sample loss
- Broad detection range: detect targets from samples ranging from a single cell to 10,000 cells
- Versatility: compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
Ideal for your qRT-PCR application
CellsDirect technology is validated for gene expression studies involving limited samples. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers).
This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available.
Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples
Specifications
| 2X | |
| Primer-Probe Detection | |
| 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system | |
| 100 | |
| Fast, Standard |
| • 10 ml Resuspension Buffer • 1 ml Lysis Enhancer • 500 µl DNase I, Amplification Grade (1 U/µl) • 160 µl 10X DNase I Buffer • 400 µl 25 mM EDTA • 100 µl SuperScript™ III RT/Platinum™ Taq Mix • 2 × 1.25 ml 2X Reaction Mix • 1 ml 50 mM MgSO4 • 2 ml DEPC-treated water • 10 µl HeLa Total RNA (10 ng/µl) • 100 µl ROX™ Reference Dye (store at -20°C in the dark) The kit is shipped on dry ice and should be stored at -20°C |
|
| Cells | |
| 1-Step qRT-PCR | |
| CellsDirect™, Platinum™, SuperScript™ |
For Research Use Only. Not for use in diagnostic procedures.