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Invitrogen™ Proteinase K Powder
Description
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.
Proteinase K Remains Active:
• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g. the presence of SDS or urea)
• In the presence of metal chelating agents (e.g. EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)
Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and Quality Testing: Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit Definition: One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Host Species | T. album |
| Packaging | Bottle |
| Content And Storage | Store dry powder in desiccant at –20°C. |
| Shipping Condition | Dry Ice |
| Quantity | 100 mg |
| Product Type | Proteinase K |
| Product Line | Ambion™ |
Safety and Handling
- Proteinase K Powder
- Danger
- Respiratory sensitiser Category 1
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- MIXTURE LIST-Contains : Proteinase
For Research Use Only. Not for use in diagnostic procedures.
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