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Eurofins DiscoverX AMPK (α1, β1, γ1) Protein, Active

Product Code. 17330754
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Quantity:
10 μg
250 μg
Unit Size:
10µg
250µg
This item is not returnable. View return policy

Product Code. 17330754

Brand: Eurofins DiscoverX 14840M

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This item is not returnable. View return policy

Description

Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1, for use in Kinase Assays.

AMPK (α1, β1, γ1) protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

GenBank AF100763 / GenBank NM_006253 / GenBank NM_002733

Specifications

Formulation mg/mL of enzyme in 50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Molecular Weight (g/mol) 64 kDa (AMPKa1), 30 kDa (AMPKb1) and 38 kDa (AMPKg1)
Quantity 250 μg
Species Human
Recombinant Recombinant
Protein Tag N-terminal 6His / untagged / untagged
Expression System Recombinant enzyme expressed in E. coli cells
Protein Form Truncated
Purity or Quality Grade Combined ≥53%
Protein AMPK (α1, β1, γ1), active
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