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Invitrogen™ Human AMPK alpha-1/beta-1/gamma-2, His Tag Recombinant Protein

Product Code. 15418903
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Quantity:
10 μg
100 μg
Unit Size:
100µg
10µg
This item is not returnable. View return policy

Product Code. 15418903

Brand: Invitrogen™ PV6238

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This item is not returnable. View return policy

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. Validated in LanthaScreen™ Kinase Binding Assay Protocols available for simple use and optimized performance Multiple pack sizes available for convenient use Related Product:LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant.

AMPK (A1/B1/G2) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.
TRUSTED_SUSTAINABILITY

Specifications

Concentration See Label
For Use With (Application) Kinase Assay
Formulation 50mM sodium phosphate with 0.1mM PMSF, 300mM NaCl, 25% glycerol, 0.2mM DTT, 150mM Imidazole and no preservative; pH 7
Molecular Weight (g/mol) 63.6-38-65 kDa
Name Human AMPK alpha-1/beta-1/gamma-2, His Tag
pH Range 7
Purification Method Purified
Quantity 10 μg
Storage Requirements -80°C, Avoid Freeze/Thaw Cycles
Regulatory Status RUO
Common Name AMPK alpha-1/beta-1/gamma-2
Protein Length 10-end
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-tag
Sequence 10-end
Content And Storage -80°C, Avoid Freeze/Thaw Cycles
Assay Entry Biochemical binding/kinetic
Kinase Group Serine/Threonine Kinase
Target Entry AMPK (A1/B1/G2)
Expression System Baculovirus
Protein Family Kinases & Inhibitors
Protein Form Recombinant
Form Liquid
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