missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Standard PCR Products
  6. Hot Start PCR Reagents and Kits
  7. Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2 

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

Product Code. 10490735 Shop All Applied Biosystems Products
Change view
Click to view available options
Quantity:
1000 Units
25,000 Units
250 Units
3000 Units
5000 Units
Unit Size:
1000 units
3000 units
5000 units
Each
5 product options available for selection
Product selection table with 5 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10490735 250 Units Each
10685095 1000 Units 1000 units
10428444 3000 Units 3000 units
10360545 5000 Units 5000 units
10640796 25,000 Units Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 5 options available.
5 options
This item is not returnable. View return policy
Product Code. 10490735 Supplier Applied Biosystems™ Supplier No. 4311806

Please to purchase this item. Need a web account? Register with us today!

277.44 EUR valid until 2026-02-28
BEST PRICE on Promo! Use promo code "28033" to get your promotional price.
This item is not returnable. View return policy

Description

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. This modified enzyme is provided in an inactive state and upon thermal activation the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Features

  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp 10X PCR Gold Buffer with MgCl2

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is limited risk of biological contamination.

GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

Notes

  • AmpliTaq Gold 360 DNA Polymerase is available for even better PCR performance.
  • AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

Order Info

Shipping condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 5 U/μL
Content And Storage • AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL
• GeneAmp 10X Gold Buffer, 1 x 1.5 mL
• 25 mM MgCl2, 1 x 1.5 mL

Store at -15°C to -30°C.
Format Tube
GC-Rich PCR Performance Low
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Quantity 250 Units
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR
Fidelity (vs. Taq) 1X
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.