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Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase
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Product Code. 10035264 Shop All Applied Biosystems Products
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Código de producto. 10035264 Proveedor Applied Biosystems™ N.º de proveedor 4398818
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Descripción

Includes

AmpliTaq 360 DNA Polymerase is supplied at 5U/μL with 10X AmpliTaq 360 PCR Buffer, 25mM M2Cl2, and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl2, and 360 GC Enhancer is supplied with each 250U of enzyme. Storage Buffer Enzyme is supplied in 20mM Tris, pH 9.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% (w/v) Tween 20, 50% (v/v) glycerol. Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq DNA Polymerase, AmpliTaq 360 DNA Polymerase is purified by an additional proprietary separation process that reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and, when combined with the proprietary AmpliTaq 360 Buffer Kit, promotes the amplification of a variety of templates, including those from bacterial and human genomes.

Features

  • Optimized for the broadest range of targets, from everyday to challenging
  • Unmatched sensitivity and yield
  • Market-leading GC enhancer for robust amplification of GC-rich sequences
  • Achieves the highest quality sequencing data

Notes

  • DreamTaq DNA Polymerase is recommended for superior PCR performance.

Información de órdenes

Approved for shipment at Room Temperature or on Wet Ice

TRUSTED_SUSTAINABILITY

Especificaciones

Concentration 5 U/μL
Content And Storage • AmpliTaq 360 DNA Polymerase (5 U/μL), 50 μL
• 10X AmpliTaq 360 PCR Buffer, 1.5 mL
• 25 mM MgCl2, 1.5 mL
• 360 GC Enhancer, 1.5 mL

Store at -15°C to -25°C.
Product is guaranteed up to 12 months as indicated by the expiration date.
Format Stand-alone enzyme
GC-Rich PCR Performance Medium
Polymerase AmpliTaq 360 DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Quantity 250 Units
Shipping Condition Room Temp or Wet Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
Hot Start No
No. of Reactions 200 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.

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