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  7. Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II 

Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

Product Code. 10762507 Shop All Applied Biosystems Products
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Includes:
Buffer I
Buffer II
Quantity:
1,000 units
1000 Units
25,000 units
250 Units
3,000 units
5,000 units
Unit Size:
Each
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Product Code. 10762507

Brand: Applied Biosystems™ N8080153

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1459.25 EUR valid until 2025-12-19
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This item is not returnable. View return policy

Description

Includes

Contains 1000 units of AmpliTaq DNA Polymerase, Buffer II, and MgCl2 Solution.

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

AmpliTaq DNA Polymerase is a 94-kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process, and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial and lot-to-lot.

AmpliTaq DNA Polymerase is available with GeneAmp10X PCR Buffer I or with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

Reliable and robust PCR

The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications.

Features

  • The most thoroughly characterized enzyme available for the PCR process
  • Excellent thermal activity profile makes it good for PCR applications
  • QC-tested to guarantee reproducible results

Notes

  • For superior PCR performance, we recommend DreamTaq DNA Polymerase.

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 5 U/μL
Content And Storage • AmpliTaq DNA Polymerase (5 U/μL), 12 x 50 μL
• GeneAmp 10X PCR Buffer II, 12 x 1.5 mL
• 25 mM MgCl2, 12 x 1.5 mL

Store at -20°C.
Format Stand-alone enzyme
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Quantity 3,000 units
Shipping Condition Dry Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
Hot Start No
No. of Reactions 2400 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA
Includes Buffer II
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For Research Use Only. Not for use in diagnostic procedures.

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