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Applied Biosystems™ MeltDoctor™ HRM Reagent Kit

Product Code. 10648095 Shop All Applied Biosystems Products
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Product Code. 10648095 Supplier Applied Biosystems™ Supplier No. 4425557

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Description

Includes

AmpliTaq Gold™ 360 DNA Polymerase, AmpliTaq Gold 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, MeltDoctor HRM Dye (20X)

Provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis.

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

This kit includes:

  • AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets
  • GeneAmp™ dNTP Blend
  • MeltDoctorHRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

Gene Expression Analysis & Genotyping, Genetic Variation Analysis Using Real-Time PCR, Genotyping & Genomic Profiling, High Resolution Melting (HRM) Analysis, Methylation Analysis, Methylation-Sensitive HRM Analysis, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR)

Order Info

Guaranteed minimum shelf life is 60 days.

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage This kit includes: AmpliTaq Gold™ 360 DNA Polymerase (2 x 50 μL, each at 5 U/μL), AmpliTaq Gold™ 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-μL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction.

Store at -15° to -25°

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method HRM
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq Gold 360 DNA Polymerase
Technique HRM (High Resolution Melting) Analysis, Mutation Scanning
For Use With (Equipment) 7500 Fast System, 7900HT Fast System
Genotyping Target SNPs (Unknown or Numerous), Epigenetic Targets
Label or Dye High Resolution Melting (HRM), SYTO 9
Product Line MeltDoctor
Product Type Hrm Reagent Kit
Quantity 1 kit
For Use With (Application) Real Time PCR (qPCR)
Sample Type DNA (Genomic), cDNA
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For Research Use Only. Not for use in diagnostic procedures.

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