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Applied Biosystems™ TaqMan™ Universal PCR Master Mix

Product Code. 10594625 Shop All Applied Biosystems Products
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Quantity:
1 x 5 mL
2 x 5 mL
5 x 5 mL
10 x 5 mL
1 x 50 mL
Tamaño de la unidad:
1 set
2000 reactions
50mL
Each
Pack of 10
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Código de producto. Quantity Tamaño de la unidad
10594625 10 x 5 mL Pack of 10
10733457 1 x 5 mL Each
10437304 5 x 5 mL 1 set
10556365 2 x 5 mL 1 set
10505215 10 x 5 mL 50mL
10675795 1 x 50 mL 2000 reactions
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Código de producto. 10594625 Proveedor Applied Biosystems™ N.º de proveedor 4305719
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Descripción

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.

• Contains AmpliTaq Gold DNA Polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq DNA Polymerase
• Includes a passive internal reference based on proprietary ROX dye, for superb precision on Applied Biosystems real-time PCR instruments
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• AmpErase UNG protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination
• Rapid assay development guidelines are provided to minimize optimization time

Alternative product: Try TaqMan Fast Advanced Master Mix (Cat. No. 4444963), our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

Información de órdenes

Guaranteed minimum shelf life is 60 days.

TRUSTED_SUSTAINABILITY

Especificaciones

Content And Storage Supplied at 2X concentration. The mix contains AmpliTaq Gold™ DNA Polymerase, Uracil-DNA Glycosylase, dTNPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains 10 boxes with one 5 mL tube each, sufficient for 2000 reactions at the 50 μL total volume. Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Kit Type 10 Pack
Passive Reference Dye ROX (Pre-mixed)
Product Line TaqMan
Product Type Universal PCR Master Mix
Quantity 10 x 5 mL
For Use With (Application) Gene Expression, Genotyping, miRNA analysis
Sample Type DNA (Genomic), cDNA
Volume 50 mL
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Frequently Asked Questions (FAQs)

What master mix can I use with the TaqMan SNP Genotyping Assays?

We recommend using either the TaqPath ProAmp Master Mix or the TaqMan Genotyping Master Mix. The TaqMan Genotyping Master Mix has the advantage of proven performance with up to 3 days of pre- and post-PCR stability, allowing you to set up plates ahead of time or read the plates later (see the data here, https://tools.thermofisher.com/content/sfs/brochures/cms_039236.pdf) while the TaqPath ProAmp Master Mix can handle samples that may have inhibitors present.

For Research Use Only. Not for use in diagnostic procedures.

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